验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, PC-3 cells, HepG2 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26023-1-AP targets ZCCHC13 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | ZCCHC13 fusion protein Ag22050 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger, CCHC domain containing 13 |
| 别名 | Cnbp2, ZCCHC13, ZNF9L |
| 计算分子量 | 166 aa, 18 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC021176 |
| 基因名称 | ZCCHC13 |
| Gene ID (NCBI) | 389874 |
| RRID | AB_3669517 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WW36 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZCCHC13 (zinc-finger CCHC-type containing 13) is a member of a family of zinc-finger CCHC-type containing DNA/RNA-binding proteins that are involved in mRNA transcription and protein translation. Downregulation of ZCCHC13 was shown to be associated with spermatogenesis failure in patients with non-obstructive azoospermia (NOA)(PMID: 29531833). It is reported that ZCCHC13 overexpression might be related to global DNA hypomethylation and promote hepatocellular carcinoma (HCC)(PMID: 23891108).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZCCHC13 antibody 26023-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |