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Z-DEVD-FMK

CAS号

210344-95-9

分子式

C30H41FN4O12

主要靶点

Caspase

仅限科研使用

Cat No : CM01198

Print datasheet

Synonyms

Caspase|Caspase-3|Caspase-3 Inhibitor|ZDEVDFMK|Z-DEVD-FMK|Z DEVD FMK|inhibit|Inhibitor

验证数据展示

  • CAS No.: 210344-95-9

    Z-DEVD-FMK
    CAS#: 210344-95-9

产品信息

CAS号 210344-95-9
分子式 C30H41FN4O12
主要靶点 Caspase
主要通路 凋亡|蛋白酶体
分子量 668.66
纯度 99.69%， 此纯度可做参考，具体纯度与批次有关系，可咨询客服
储存条件 Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
别名 Caspase|Caspase-3|Caspase-3 Inhibitor|ZDEVDFMK|Z-DEVD-FMK|Z DEVD FMK|inhibit|Inhibitor

靶点活性

Caspase-3:18 μM.

体内活性

方法：为测试体内活性，将 Z-DEVD-FMK (1.8 mg/kg in ethanol and freshly diluted in PBS containing 2% Tween-80) 腹腔注射给 C57BL/6 小鼠，30 min 后注射 CPT-11 (350 mg/kg)。Z-DEVD-FMK 继续给药三天，每天一次。 结果：Caspase-3 抑制剂 Z-DEVD-FMK 减弱 PT-11 诱导的 GATA6 缺失小鼠腹腔巨噬细胞。[3]

体外活性

方法：v-K-ras转化的正常大鼠肾脏细胞 KNRK 用 SCH56582 (20 ?μM) 和 Z-DEVD-FMK (20-50 ?μM) 处理 24 h，使用 trypan blue 检测细胞死亡。 结果：添加 50 μM Z-DEVD-fmk 导致细胞凋亡抑制 >70%。[1] 方法：人多发性骨髓瘤细胞 KM3 用 betulinic acid (15 ?μg/mL) 和 Z-DEVD-FMK (50 ?mol/L) 处理 24 h，使用 Western Blot 检测靶点蛋白表达水平。 结果：Z-DEVD-VMK 减轻了 betulinic acid 诱导的 caspase 3 的激活。Z-DEVD-FMK 显著阻断 PARP 的切割。[2]

溶解度

Ethanol:< 1 mg/mL (insoluble or slightly soluble);H2O:< 1 mg/mL (insoluble or slightly soluble);DMSO:50 mg/mL (74.78 mM)

细胞实验

N27 cells are incubated with 100 μM 6-OHDA for 24 h or 300 μM MPP+ for 36 h in the presence or absence of 50 μM Z-DEVD-FMK and cell death is determined by MTT (3-(4,5-dimethylthiazol-3-yl)-2,5-diphenyl tetrazolium bromide) assay, which is widely used to assess cell viability. After treatment, the cells are incubated in serum-free medium containing 0.25 mg/ml MTT for 3 h at 37°C. Formation of formazan from tetrazolium is measured at 570 nm with a reference wavelength at 630 nm using a SpectraMax microplate reader.(Only for Reference)

参考文献

1.Suzuki N, et al. Farnesyltransferase inhibitors induce cytochrome c release and caspase 3 activation preferentially in transformed cells. Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15356-61.
2.Zhu Q, Ding L, Zi Z, et al. Viral-Mediated AURKB Cleavage Promotes Cell Segregation and Tumorigenesis[J]. Cell reports. 2019 Mar 26;26(13):3657-3671.e5.
3.Yang LJ, et al. Betulinic acid inhibits autophagic flux and induces apoptosis in human multiple myeloma cells in vitro. Acta Pharmacol Sin. 2012 Dec;33(12):1542-8.
4.Huang MY, et al. Chemotherapeutic agent CPT-11 eliminates peritoneal resident macrophages by inducing apoptosis. Apoptosis. 2016 Feb;21(2):130-42.
5.Meguro T, et al. Stroke. 2001, 32(2), 561-566.
6.Kanthasamy AG, et al. Free Radic Biol Med. 2006, 41(10), 1578-1589.
7.Zhang Y, Fan B Y, Pang Y L, et al. Neuroprotective effect of deferoxamine on erastininduced ferroptosis in primary cortical neurons[J]. Neural Regeneration Research. 2020, 15(8): 1539.
8.Zhang Y, Fan B Y, Pang Y L, et al. Neuroprotective effect of deferoxamine on erastin-induced ferroptosis in primary cortical neurons[J]. Neural regeneration research. 2020, 15(8): 1539.
9.Wang X Y, Wu K H, Pang H L, et al. Study on the Role of Cytc in Response to BmNPV Infection in Silkworm, Bombyx mori (Lepidoptera)[J]. International journal of molecular sciences. 2019, 20(18): 4325.
10.Liu X, Song X, Luan D, et al. Real-time in situ Visualizing the Sequential Activation of Caspase Cascade Using a Multi-color Au-Se Bonding Fluorescent Nanoprobe[J]. Analytical chemistry. 2019 May 7;91(9):5994-6002.

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