• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 31228-1-AP

    WB analysis using 31228-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31228-1-AP (YARS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 31228-1-AP

    WB analysis of A431 using 31228-1-AP

    WB result of YARS antibody (31228-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YARS transfected A431 cells.

  • IHC staining of human stomach cancer using 31228-1-AP

    IHC staining of human stomach cancer using 31228-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31228-1-AP (YARS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 31228-1-AP

    IHC staining of human ovary cancer using 31228-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31228-1-AP (YARS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

YARS Polyclonal antibody

YARS Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31228-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, pig

应用

WB, IHC, ELISA

别名

YARS1, CMTDIC, TYRRS, YRS, YTS

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA431 cells, U2OS cells, pig pancreas tissue, pig tonsil tissue
Positive IHC detected inhuman stomach cancer tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31228-1-AP targets YARS in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag35148

Product name: Recombinant human YARS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 400-528 aa of BC016689

Sequence: RTVVSGLVQFVPKEELQDRLVVVLCNLKPQKMRGVESQGMLLCASIEGINRQVEPLDPPAGSAPGEHVFVKGYEKGQPDEELKPKKKVFEKLQADFKISEECIAQWKQTNFMTKLGSISCKSLKGGNIS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tyrosyl-tRNA synthetase
别名 YARS1, CMTDIC, TYRRS, YRS, YTS
计算分子量 59 kDa
观测分子量60 kDa
GenBank蛋白编号BC016689
基因名称 YARS
Gene ID (NCBI) 8565
RRIDAB_3669909
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP54577
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tyrosyl-tRNA synthetase (YARS) is responsible for tyrosyl-tRNA aminoacylation. The functional enzyme exists as a homodimer, has potent leukocyte and monocyte chemotaxis activity, and stimulates production of myeloperoxidase, tumor necrosis factor-α, and tissue factor. A shift in the monomer:dimer equilibrium could result in dysregulation of the cytokines function. (PMID: 33490854)

实验方案

Product Specific Protocols
IHC protocol for YARS antibody 31228-1-APDownload protocol
WB protocol for YARS antibody 31228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...