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验证数据展示

  • IHC staining of human intrahepatic cholangiocarcinoma using 25204-1-AP (same clone as 25204-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 25204-1-AP (same clone as 25204-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25204-1-AP (WIPI1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25204-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 25204-1-AP (same clone as 25204-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 25204-1-AP (same clone as 25204-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25204-1-AP (WIPI1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25204-1-PBS in a different storage buffer formulation.

  • WB analysis of SW 1990 using 25204-1-AP (same clone as 25204-1-PBS)

    WB analysis of SW 1990 using 25204-1-AP (same clone as 25204-1-PBS)

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 25204-1-AP (WIPI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25204-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

WIPI1 Polyclonal antibody, PBS Only

WIPI1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25204-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

WIPI-1, WIPI 49 kDa, WIPI 1, WD repeat domain phosphoinositide-interacting protein 1, Atg18 protein homolog

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

25204-1-PBS targets WIPI1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 WIPI1 fusion protein Ag18461 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeat domain, phosphoinositide interacting 1
别名 WIPI-1, WIPI 49 kDa, WIPI 1, WD repeat domain phosphoinositide-interacting protein 1, Atg18 protein homolog
计算分子量 446 aa, 49 kDa
观测分子量 49 kDa
GenBank蛋白编号BC039867
基因名称 WIPI1
Gene ID (NCBI) 55062
RRIDAB_2879958
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5MNZ9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WIPI1, or WD repeat domain, phosphoinositide-interacting protein 1, is a member of the WD40 repeat family of proteins, which are key components of many essential biological functions. WIPI1 is involved in the regulation of autophagy, a cellular process critical for maintaining cellular integrity and recycling intracellular proteins, lipids, and organelles. WIPI1 is characterized by its 7-bladed β-propeller structure and contains a conserved motif for interaction with phospholipids, specifically binding to phosphoinositides. This interaction is crucial for its function in autophagy, where it acts as an effector of phosphatidylinositol 3-phosphate (PtdIns3P). WIPI1 and WIPI2 are among the first proteins to be recruited during autophagy, with WIPI2 interacting with components of the ULK1/2 complex and PtdIns3P, tethering the phagophore to the endoplasmic reticulum (ER). WIPI1 supports the function of WIPI2 in recruiting the ATG16L complex, which is essential for LC3 lipidation and autophagosome formation.