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验证数据展示

  • WB analysis using 22047-1-AP

    WB analysis using 22047-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22047-1-AP (WIPF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 22047-1-AP

    IHC staining of human stomach cancer using 22047-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22047-1-AP (WIPF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 22047-1-AP

    IHC staining of human stomach cancer using 22047-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22047-1-AP (WIPF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

WIPF1 Polyclonal antibody

WIPF1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22047-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Protein PRPL 2, PRPL 2, WASP interacting protein, WASPIP, WIP, WIPF1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHL-60 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22047-1-AP targets WIPF1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag16975

Product name: Recombinant human WIPF1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 14-238 aa of BC110288

Sequence: TFALANTEKPTLNKTEQAGRNALLSDISKGKKLKKTVTNDRSAPILDKPKGAGAGGGGGGFGGGGGFGGGGGGGGGGSFGGGGPPGLGGLFQAGMPKLRSTANRDNDSGGSRPPLLPPGGRSTSAKPFSPPSGPGRFPVPSPGHRSGPPEPQRNRMPPPRPDVGSKPDSIPPPVPSTPRPIQSSLHNRGSPPVPGGPRQPSPGPTPPPFPGNRGTALGGGSIRQS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WAS/WASL interacting protein family, member 1
别名 Protein PRPL 2, PRPL 2, WASP interacting protein, WASPIP, WIP, WIPF1
计算分子量403aa,40 kDa; 503aa,51 kDa
观测分子量 51 kDa
GenBank蛋白编号BC110288
基因名称 WIPF1
Gene ID (NCBI) 7456
RRIDAB_3085682
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43516
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WASP interacting protein family member 1(WIPF1), also known as WIP, is a ubiquitously expressed proline-rich multidomain protein and plays a role in the reorganization of the actin cytoskeleton. Mutations in the WIPF1 binding site of the Wiskott-Aldrich syndrome protein or in WIPF1 itself cause Wiskott-Aldrich syndrome, which predisposes people to cancer, such as leukemia and lymphoma(PMID: 27863429).

实验方案

Product Specific Protocols
IHC protocol for WIPF1 antibody 22047-1-APDownload protocol
WB protocol for WIPF1 antibody 22047-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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