验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, mouse cerebellum tissue, rat kidney tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IP | See 1 publications below |
产品信息
29997-1-AP targets WDR73 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | WDR73 fusion protein Ag31818 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | WD repeat domain 73 |
| 别名 | WD repeat-containing protein 73, WD repeat domain 73, HSPC264 |
| 观测分子量 | 41 kDa |
| GenBank蛋白编号 | BC063392 |
| 基因名称 | WDR73 |
| Gene ID (NCBI) | 84942 |
| RRID | AB_3086206 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6P4I2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WDR73 contains multiple WD40 repeat domains that function as scaffolds for the assembly of protein complexes. WDR73 was present in the brain and kidney and was located diffusely in the cytoplasm during interphase but relocalized to spindle poles and astral microtubules during mitosis. WDR73 interacts with microtubules to regulate cell cycle progression, proliferation, and survival in the brain and kidney(PMID: 26070982). Reduced expression of WDR73 results in abnormalities in the size and morphology of the nucleus. Mutations in WDR73 have been associated with Galloway-Mowat syndrome, which is a rare autosomal recessive disorder that affects both the central nervous system and the kidney (PMID: 25466283).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WDR73 antibody 29997-1-AP | Download protocol |
| IHC protocol for WDR73 antibody 29997-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |