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验证数据展示

  • IHC staining of human breast cancer using 26175-1-AP

    IHC staining of human breast cancer using 26175-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26175-1-AP (VWA5A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 26175-1-AP

    IHC staining of human breast cancer using 26175-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26175-1-AP (VWA5A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

VWA5A Polyclonal antibody

VWA5A Polyclonal Antibody for IHC, ELISA
Cat No. 26175-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, ELISA

别名

BCSC1, Breast cancer suppressor candidate 1, von Willebrand factor A domain containing 5A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman breast cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26175-1-AP targets VWA5A in IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag22010

Product name: Recombinant human VWA5A protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-415 aa of BC001234

Sequence: MVHFCGLLTLHREPVPLKSISVSVNIYEFVAGVSATLNYENEEKVPLEAFFVFPMDEDSAVYSFEALVDGKKIVAELQDKMKARTNYEKAISQGHQAFLLEGDSSSRDVFSCNVGNLQPGSKAAVTLKYVQELPLEADGALRFVLPAVLNPRYQFSGSSKDSCLNVKTPIVPVEDLPYTLSMVATIDSQHGIEKVQSNCPLSPTEYLGEDKTSAQVSLAAGHKFDRDVELLIYYNEVHTPSVVLEMGMPNMKPGHLMGDPSAMVSFYPNIPEDQPSNTCGEFIFLMDRSGSMQSPMSSQDTSQLRIQAAKETLILLLKSLPIGCYFNIYGFGSSYEACFPESVKYTQQTMEEALGRVKLMQADLGGTEILAPLQNIYRGPSIPGHPLQLFVFTDGEVTDTFSVIKEVRINRQKHR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 von Willebrand factor A domain containing 5A
别名 BCSC1, Breast cancer suppressor candidate 1, von Willebrand factor A domain containing 5A
计算分子量 786 aa, 86 kDa
GenBank蛋白编号BC001234
基因名称 VWA5A
Gene ID (NCBI) 4013
RRIDAB_3669526
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00534
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VWA5A, also known as BCSC1 or LOH11CR2A, may function as a tumor suppressor in tumorigenesis. The lower expression of VWA5A may serve as a potential biomarker for metastasis-prone breast cancers (PMID: 38291227).

实验方案

Product Specific Protocols
IHC protocol for VWA5A antibody 26175-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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