• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 30599-1-AP (same clone as 30599-1-PBS)

    WB analysis using 30599-1-AP (same clone as 30599-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30599-1-AP (VCAN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30599-1-PBS in a different storage buffer formulation.

  • IHC staining of human endometrial cancer using 30599-1-AP (same clone as 30599-1-PBS)

    IHC staining of human endometrial cancer using 30599-1-AP (same clone as 30599-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30599-1-AP (VCAN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30599-1-PBS in a different storage buffer formulation.

  • IHC staining of human endometrial cancer using 30599-1-AP (same clone as 30599-1-PBS)

    IHC staining of human endometrial cancer using 30599-1-AP (same clone as 30599-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30599-1-AP (VCAN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30599-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 30599-1-AP (same clone as 30599-1-PBS)

    IHC staining of human stomach cancer using 30599-1-AP (same clone as 30599-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30599-1-AP (VCAN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30599-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 30599-1-AP (same clone as 30599-1-PBS)

    IHC staining of human stomach cancer using 30599-1-AP (same clone as 30599-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30599-1-AP (VCAN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30599-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 30599-1-AP (same clone as 30599-1-PBS)

    IF Staining of NIH/3T3 using 30599-1-AP (same clone as 30599-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using VCAN antibody (30599-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30599-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 30599-1-AP (same clone as 30599-1-PBS)

    IF Staining of NIH/3T3 using 30599-1-AP (same clone as 30599-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using VCAN antibody (30599-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 30599-1-PBS in a different storage buffer formulation.

VCAN Polyclonal antibody, PBS Only

VCAN Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30599-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CSPG2, ERVR, GHAP, Glial hyaluronate-binding protein, Large fibroblast proteoglycan

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

30599-1-PBS targets VCAN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 VCAN fusion protein Ag30151 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 versican
别名 CSPG2, ERVR, GHAP, Glial hyaluronate-binding protein, Large fibroblast proteoglycan
计算分子量 373 kDa
观测分子量250-300 kDa, 550 kDa
GenBank蛋白编号NM_004385
基因名称 VCAN
Gene ID (NCBI) 1462
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13611
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Versican, a component of the extracellular matrix and a member of the lectican family of proteoglycans, is the largest chondroitin sulfate proteoglycan and has an important role in the development of the heart, musculoskeletal system and central nervous system (CNS). The structure of versican is characterized by an approximately 550-kDa core protein consisting of an amino-terminal (G1) hyaluronan binding domain, a carboxy-terminal (G3) domain, and two central chondroitin sulfate glycosaminoglycan attachment domains, the α-GAG and β-GAG domains. Versican has four different isoforms that differ in the central GAG attachment domain: V0 (contains both α and β GAG domains), V1 (contains only the β domain), V2 (contains only the α domain) and V3 (devoid of both GAG domains)(PMID: 26385570).