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验证数据展示

  • IHC staining of human liver cancer using 15776-1-AP (same clone as 15776-1-PBS)

    IHC staining of human liver cancer using 15776-1-AP (same clone as 15776-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 15776-1-AP (same clone as 15776-1-PBS)

    IHC staining of human liver cancer using 15776-1-AP (same clone as 15776-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

  • WB analysis using 15776-1-AP (same clone as 15776-1-PBS)

    WB analysis using 15776-1-AP (same clone as 15776-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 15776-1-AP (same clone as 15776-1-PBS)

    IP experiment of HeLa using 15776-1-AP (same clone as 15776-1-PBS)

    IP result of anti-VARS2 (IP:15776-1-AP, 4ug; Detection:15776-1-AP 1:500) with HeLa cells lysate 2400ug. This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 15776-1-AP (same clone as 15776-1-PBS)

    IF Staining of HeLa using 15776-1-AP (same clone as 15776-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VARS2 antibody (15776-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

VARS2 Polyclonal antibody, PBS Only

VARS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 15776-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

Valyl tRNA synthetase like, ValRS, Valine--tRNA ligase, mitochondrial, Valine tRNA ligase, KIAA1885

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

15776-1-PBS targets VARS2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8460

Product name: Recombinant human VARS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 168-516 aa of BC008844

Sequence: TDALRFTLCSHGVQAGDLHLSVSEVQSCRHFCNKIWNALRFILNALGEKFVPQPAEELSPSSPMDAWILSRLALAAQECERGFLTRELSLVTHALHHFWLHNLCDVYLEAVKPVLWHSPRPLGPPQVLFSCADLGLRLLAPLMPFLAEELWQRLPPRPGCPPAPSISVAPYPSACSLEHWRQPELERRFSRVQEVVQVLRALRATYQLTKARPRVLLQSSEPGDQGLFEAFLEPLGTLGYCGAVGLLPPGAAAPSGWAQAPLSDTAQVYMELQGLVDPQIQLPLLAARRYKLQKQLDSLTARTPSEGEAGTQRQQKLSSLQLELSKLDKAASHLQQLMDEPPAPGSPEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 valyl-tRNA synthetase 2, mitochondrial (putative)
别名 Valyl tRNA synthetase like, ValRS, Valine--tRNA ligase, mitochondrial, Valine tRNA ligase, KIAA1885
计算分子量 119 kDa, 57 kDa
观测分子量 118 kDa
GenBank蛋白编号BC008844
基因名称 VARS2
Gene ID (NCBI) 57176
RRIDAB_2272699
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5ST30
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VARS2(Valine--tRNA ligase, mitochondrial) is also named as KIAA1885, VARS2L, VARSL and belongs to the class-I aminoacyl-tRNA synthetase family. The deduced 993-amino acid protein has an N-terminal mitochondrial targeting signal with a predicted cleavage site after residue 29. VARS2 has characteristics of a class I mitochondrial aminoacyl-tRNA synthetase, including a classical Rossmann fold.

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