• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 15275-1-AP (same clone as 15275-1-PBS)

    WB analysis using 15275-1-AP (same clone as 15275-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15275-1-AP (VAPA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 15275-1-AP (same clone as 15275-1-PBS)

    IHC staining of human colon cancer using 15275-1-AP (same clone as 15275-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 15275-1-AP (same clone as 15275-1-PBS)

    IHC staining of human colon cancer using 15275-1-AP (same clone as 15275-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 15275-1-AP (same clone as 15275-1-PBS)

    IP experiment of HEK-293 using 15275-1-AP (same clone as 15275-1-PBS)

    IP result of anti-VAPA (IP:15275-1-AP, 3ug; Detection:15275-1-AP 1:1000) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 15275-1-AP (same clone as 15275-1-PBS)

    IF Staining of HepG2 using 15275-1-AP (same clone as 15275-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VAPA antibody (15275-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 15275-1-AP (same clone as 15275-1-PBS)

    IHC staining of mouse brain using 15275-1-AP (same clone as 15275-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 15275-1-AP (same clone as 15275-1-PBS)

    IF Staining of HepG2 using 15275-1-AP (same clone as 15275-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VAPA antibody (15275-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

VAPA Polyclonal antibody, PBS Only

VAPA Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 15275-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

33 kDa VAMP associated protein, 33 kDa VAMP-associated protein, hVAP 33, VAMP A, VAMP associated protein A

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

15275-1-PBS targets VAPA in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7393

Product name: Recombinant human VAPA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-221 aa of BC002992

Sequence: MASASGAMAKHEQILVLDPPTDLKFKGPFTDVVTTNLKLRNPSDRKVCFKVKTTAPRRYCVRPNSGIIDPGSTVTVSVMLQPFDYDPNEKSKHKFMVQTIFAPPNTSDMEAVWKEAKPDELMDSKLRCVFEMPNENDKLNDMEPSKAVPLNASKQDGPMPKPHSVSLNDTETRKLMEECKRLQGEMMKLSEENRHLRDEGLRLRKVAHSDKPGSTSTASFR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
别名 33 kDa VAMP associated protein, 33 kDa VAMP-associated protein, hVAP 33, VAMP A, VAMP associated protein A
计算分子量 28 kDa
观测分子量28-30 kDa
GenBank蛋白编号BC002992
基因名称 VAPA
Gene ID (NCBI) 9218
RRIDAB_2256991
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P0L0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VAPA (Vesicle-associated membrane protein-associated protein A), also known as VAP33, is a ubiquitously expressed integral membrane protein of the VAMP-associated protein (VAP) family. It is present in the plasma membrane and in intracellular vesicles, playing a role in vesicle trafficking.

||
New chat Able
正在加载，请稍候...