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验证数据展示

  • WB analysis using 15275-1-AP (same clone as 15275-1-PBS)

    WB analysis using 15275-1-AP (same clone as 15275-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15275-1-AP (VAPA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 15275-1-AP (same clone as 15275-1-PBS)

    IHC staining of human colon cancer using 15275-1-AP (same clone as 15275-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 15275-1-AP (same clone as 15275-1-PBS)

    IHC staining of human colon cancer using 15275-1-AP (same clone as 15275-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15275-1-AP (VAPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 15275-1-AP (same clone as 15275-1-PBS)

    IP experiment of HEK-293 using 15275-1-AP (same clone as 15275-1-PBS)

    IP result of anti-VAPA (IP:15275-1-AP, 3ug; Detection:15275-1-AP 1:1000) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 15275-1-AP (same clone as 15275-1-PBS)

    IF Staining of HepG2 using 15275-1-AP (same clone as 15275-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VAPA antibody (15275-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 15275-1-AP (same clone as 15275-1-PBS)

    IF Staining of HepG2 using 15275-1-AP (same clone as 15275-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VAPA antibody (15275-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 15275-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

VAPA Polyclonal antibody, PBS Only

VAPA Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 15275-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

VAMP-A, VAMP associated protein A, VAMP A, hVAP 33, 33 kDa VAMP-associated protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

15275-1-PBS targets VAPA in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 VAPA fusion protein Ag7393 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
别名 VAMP-A, VAMP associated protein A, VAMP A, hVAP 33, 33 kDa VAMP-associated protein
计算分子量 28 kDa
观测分子量28-30 kDa
GenBank蛋白编号BC002992
基因名称 VAPA
Gene ID (NCBI) 9218
RRIDAB_2256991
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P0L0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VAPA (Vesicle-associated membrane protein-associated protein A), also known as VAP33, is a ubiquitously expressed integral membrane protein of the VAMP-associated protein (VAP) family. It is present in the plasma membrane and in intracellular vesicles, playing a role in vesicle trafficking.