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验证数据展示

  • WB analysis using 22268-1-AP (same clone as 22268-1-PBS)

    WB analysis using 22268-1-AP (same clone as 22268-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 22268-1-AP (same clone as 22268-1-PBS)

    IHC staining of human liver using 22268-1-AP (same clone as 22268-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 22268-1-AP (same clone as 22268-1-PBS)

    IHC staining of human liver using 22268-1-AP (same clone as 22268-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 22268-1-AP (same clone as 22268-1-PBS)

    IF Staining of HepG2 using 22268-1-AP (same clone as 22268-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VAMP7/TI-VAMP antibody (22268-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 22268-1-AP (same clone as 22268-1-PBS)

    IP experiment of mouse brain using 22268-1-AP (same clone as 22268-1-PBS)

    IP result of anti-VAMP7/TI-VAMP (IP:22268-1-AP, 4ug; Detection:22268-1-AP 1:1000) with mouse brain tissue lysate 3440ug. This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

VAMP7/TI-VAMP Polyclonal antibody, PBS Only

VAMP7/TI-VAMP Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 22268-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

VAMP7, TI VAMP, Tetanus-insensitive VAMP, Tetanus insensitive VAMP, Synaptobrevin-like protein 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

22268-1-PBS targets VAMP7/TI-VAMP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vesicle-associated membrane protein 7
别名 VAMP7, TI VAMP, Tetanus-insensitive VAMP, Tetanus insensitive VAMP, Synaptobrevin-like protein 1
计算分子量 25 kDa
观测分子量 20-25 kDa
GenBank蛋白编号NM_005638
基因名称 VAMP7
Gene ID (NCBI) 6845
RRIDAB_11182718
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51809
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VAMP7, also named SYBL1 and TI-VAMP, belongs to the synaptobrevin family. It is involved in the targeting and/or fusion of transport vesicles to their target membrane during the transport of proteins from the early endosome to the lysosome. VAMP7 is required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. It is necessary for calcium-regulated lysosomal exocytosis. VAMP7 is involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. It is required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. It is also required for focal exocytosis of late endocytic vesicles during phagosome formation. The antibody is specific to VAMP7.