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Species Reactivity
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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 30239-1-AP

    WB analysis using 30239-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30239-1-AP (USP50 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

USP50 Polyclonal antibody

USP50 Polyclonal Antibody for WB, ELISA
Cat No. 30239-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

Ubiquitin-specific peptidase 50, Ubiquitin carboxyl-terminal hydrolase 50, EC:3.4.19.12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30239-1-AP targets USP50 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 USP50 fusion protein Ag31275 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 50
别名 Ubiquitin-specific peptidase 50, Ubiquitin carboxyl-terminal hydrolase 50, EC:3.4.19.12
计算分子量 339 aa, 39 kDa
观测分子量40 kDa
GenBank蛋白编号BC146493
基因名称 USP50
Gene ID (NCBI) 373509
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ70EL3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP50 (Ubiquitin Specific Peptidase 50) is a deubiquitinating enzyme that belongs to the USP family. It plays a key role in DNA replication, genome stability and cell cycle regulation. Recent studies have found that USP50 maintains the stability of the DNA replication process and telomere integrity by regulating the use of nuclease and deconjugating enzymes. During replication, USP50 was able to inhibit the harmful DNA2 nuclease activity and substitute the use of RecQ deconjugase, thereby preventing replication fork stalling and DNA damage. In addition, USP50 plays a role in the cell cycle G2/M phase transition, assembly of the NLRP3 inflammasome complex, and organization of nuclear speckles. Its loss of function leads to DNA double-strand breaks and telomere instability in cells under replication stress. These findings provide new insights into the understanding of the pathogenesis of genetic diseases and may offer potential targets for the treatment of diseases such as cancer and premature aging.

实验方案

Product Specific Protocols
WB protocol for USP50 antibody 30239-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols