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验证数据展示

  • WB analysis of mouse lung using 15158-1-AP (same clone as 15158-1-PBS)

    WB analysis of mouse lung using 15158-1-AP (same clone as 15158-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 15158-1-AP (same clone as 15158-1-PBS)

    WB analysis of A549 using 15158-1-AP (same clone as 15158-1-PBS)

    WB result of USP5 antibody (15158-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP5 transfected A549 cells. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 15158-1-AP (same clone as 15158-1-PBS)

    IP experiment of mouse brain using 15158-1-AP (same clone as 15158-1-PBS)

    IP result of anti-USP5 (IP:15158-1-AP, 4ug; Detection:15158-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

USP5 Polyclonal antibody, PBS Only

USP5 Polyclonal Antibody for WB, IP, ELISA
Cat No. 15158-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

15158-1-PBS targets USP5 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 USP5 fusion protein Ag7252 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 5 (isopeptidase T)
别名
计算分子量 96 kDa
观测分子量 95-105 kDa
GenBank蛋白编号BC005139
基因名称 USP5
Gene ID (NCBI) 8078
RRIDAB_2213856
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP45974
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

USP5, also named as ISOT, belongs to the peptidase C19 family. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition. USP5 is a potential target for p53 activating therapeutic agents for the treatment of cancer(PMID: 19098288). It is a novel proteasome associated protein(PMID: 19182904). USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers.