UNC2250

Mer:1.7 nM

根据体内PK实验，UNC2250具有中等的半衰期、清除率和分布体积，同时具备合理的口服生物利用度和良好的溶解性。[1]

DMSO:25 mg/mL (56.74 mM);20% Cremophor EL:5 mg/mL (11.35 mM)

BT-12 rhabdoid tumor cells (10?000 cells) are cultured in 2.0 mL of 0.35% soft agar containing 0.5× RPMI medium, 7.5% FBS, and the indicated concentrations of 10 or DMSO vehicle only and overlaid with 0.5 mL of 1× RPMI medium containing 15% FBS and 10 or DMSO vehicle only. Medium and 10 or vehicle are refreshed 2 times per week. Colonies were stained with thiazolyl blue tetrazolium bromide and counted after 3 weeks. Colo699 NSCLC cells (15?000 cells) are cultured in 1.5 mL of 0.35% soft agar containing 1× RPMI medium and 10% FBS and overlaid with 2.0 mL of 1× RPMI medium containing 10% FBS and the indicated concentrations of 10 or DMSO vehicle only. Medium and 10 or vehicle are refreshed 3 times per week. Colonies are stained with nitrotetrazolium blue chloride and counted after 2 weeks.(Only for Reference)