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验证数据展示

  • WB analysis of mouse liver using 20363-1-AP

    WB analysis of mouse liver using 20363-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20363-1-AP (UBN1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 20363-1-AP

    IHC staining of human cervical cancer using 20363-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20363-1-AP (UBN1-Specific antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human cervical cancer using 20363-1-AP

    IHC staining of human cervical cancer using 20363-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20363-1-AP (UBN1-Specific antibody) at dilution of 1:50 (under 40x lens).

UBN1-Specific Polyclonal antibody

UBN1-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20363-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

HIRA binding protein, Protein VT4, ubinuclein 1, UBN1, UBN1-Specific, VT, VT4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, MCF-7 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20363-1-AP targets UBN1-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubinuclein 1
别名 HIRA binding protein, Protein VT4, ubinuclein 1, UBN1, UBN1-Specific, VT, VT4
计算分子量 121 kDa
观测分子量 120-140 kDa
GenBank蛋白编号NM_016936
基因名称 UBN1
Gene ID (NCBI) 29855
RRIDAB_10694818
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NPG3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBN1, also named as Protein VT4, belongs to the ubinuclein family. UBN1 acts as a novel regulator of senescence. It is involved in the formation of senescence-associated heterochromatin foci (SAHF), which represses expression of proliferation-promoting genes. UBN1 binds to proliferation-promoting genes. It may be required for replication-independent chromatin assembly. The antibody is specific to UBN1.

实验方案

Product Specific Protocols
WB protocol for UBN1-Specific antibody 20363-1-APDownload protocol
IHC protocol for UBN1-Specific antibody 20363-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Stem Cell

PHB Associates with the HIRA Complex to Control an Epigenetic-Metabolic Circuit in Human ESCs.

Authors - Zhexin Zhu
View Article