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验证数据展示

  • WB analysis of mouse liver using 20363-1-AP

    WB analysis of mouse liver using 20363-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20363-1-AP (UBN1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 20363-1-AP

    IHC staining of human cervical cancer using 20363-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20363-1-AP (UBN1-Specific antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human cervical cancer using 20363-1-AP

    IHC staining of human cervical cancer using 20363-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20363-1-AP (UBN1-Specific antibody) at dilution of 1:50 (under 40x lens).

UBN1-Specific Polyclonal antibody

UBN1-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20363-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

HIRA binding protein, Protein VT4, ubinuclein 1, UBN1, UBN1-Specific, VT, VT4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, MCF-7 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20363-1-AP targets UBN1-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubinuclein 1
别名 HIRA binding protein, Protein VT4, ubinuclein 1, UBN1, UBN1-Specific, VT, VT4
计算分子量 121 kDa
观测分子量 120-140 kDa
GenBank蛋白编号NM_016936
基因名称 UBN1
Gene ID (NCBI) 29855
RRIDAB_10694818
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NPG3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBN1, also named as Protein VT4, belongs to the ubinuclein family. UBN1 acts as a novel regulator of senescence. It is involved in the formation of senescence-associated heterochromatin foci (SAHF), which represses expression of proliferation-promoting genes. UBN1 binds to proliferation-promoting genes. It may be required for replication-independent chromatin assembly. The antibody is specific to UBN1.

实验方案

Product Specific Protocols
WB protocol for UBN1-Specific antibody 20363-1-APDownload protocol
IHC protocol for UBN1-Specific antibody 20363-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Stem Cell

PHB Associates with the HIRA Complex to Control an Epigenetic-Metabolic Circuit in Human ESCs.

Authors - Zhexin Zhu
View Article