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验证数据展示

  • WB analysis of HeLa using 14415-1-AP (same clone as 14415-1-PBS)

    WB analysis of HeLa using 14415-1-AP (same clone as 14415-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse kidney using 14415-1-AP (same clone as 14415-1-PBS)

    IP experiment of mouse kidney using 14415-1-AP (same clone as 14415-1-PBS)

    IP result of anti-UBE2L3 (IP:14415-1-AP, 3ug; Detection:14415-1-AP 1:2000) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 14415-1-AP (same clone as 14415-1-PBS)

    IHC staining of human lung cancer using 14415-1-AP (same clone as 14415-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14415-1-AP (UBE2L3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBE2L3 antibody included
  • IHC staining of human lung cancer using 14415-1-AP (same clone as 14415-1-PBS)

    IHC staining of human lung cancer using 14415-1-AP (same clone as 14415-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14415-1-AP (UBE2L3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBE2L3 antibody included
  • Featured Product
  • KD/KO验证

UBE2L3 Polyclonal antibody, PBS Only

UBE2L3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 14415-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy UBE2L3 Ready-To-Use IHC Kit. UBE2L3 primary antibody included.

产品信息

14415-1-PBS targets UBE2L3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5758

Product name: Recombinant human UBE2L3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-154 aa of BC053368

Sequence: MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin-conjugating enzyme E2L 3
别名
计算分子量 18 kDa
观测分子量 18 kDa
GenBank蛋白编号BC053368
基因名称 UBE2L3
Gene ID (NCBI) 7332
RRIDAB_2210891
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP68036
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UBE2L3(Ubiquitin-conjugating enzyme E2 L3) is also named as UBCE7, UBCH7 and belongs to the ubiquitin-conjugating enzyme family. It regulates nuclear hormone receptors transcriptional activity and may play a role in myelopoiesis.UBE2L3 is associated with susceptibility to systemic lupus erythematosus (SLE) and rheumatoid arthritis in European ancestry populations(PMID:22045845).

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