Beta Tubulin Monoclonal antibody

Beta Tubulin Monoclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, nematode, pig, zebrafish and More (3)

Applications

IF/ICC, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1D4A4

Cat No : 66240-1-Ig

Print datasheet

Synonyms

beta tubulin, B-tubulin, MC1R, TUBB, Tubulin-beta



经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, MCF-7 cells, NCCIT cells, HT-1080 cells, LNCaP cells, ROS1728 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman brain tissue, human breast cancer tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TUBB3 Ready-To-Use IHC Kit. TUBB3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 750 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66240-1-Ig targets Beta Tubulin in WB, IHC, IF/ICC, IP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat, nematode, pig, zebrafish samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA Application Description
Cited ApplicationsWB, IHC, IP, Cell treatment, IF
Tested Reactivity human, mouse, rat, nematode, pig, zebrafish
Cited Reactivityhuman, mouse, rat, pig, rabbit, chicken, zebrafish, sheep
Immunogen Beta Tubulin fusion protein Ag0117 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name tubulin, beta 3
Synonyms beta tubulin, B-tubulin, MC1R, TUBB, Tubulin-beta
Calculated Molecular Weight 450 aa, 50 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC000748
Gene Symbol TUBB3
Gene ID (NCBI) 10381
RRIDAB_2881629
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13509
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The alpha and beta tubulins (+/- 55 kDa MW) are homologous but are not identical. Beta tubulins have been widely used as loading control.

 

What is the molecular weight of beta-tubulin? Are there any isoforms of beta-tubulin?

The molecular weight of tubulin is 50-52 kDa. Humans have eight beta-tubulin isotypes, encoded by different genes, that differ in their C-terminal sequences. They have different tissue expression profiles and can rise to microtubules of different properties (PMID: 20191564).

 

How to use beta-tubulin as a loading control

Beta-tubulin is one of the most commonly used references as a loading control for cell lysates in western blotting. It is abundantly expressed across various tissues and developmental stages and highly conserved across species. However, since some variability has been observed in the expression levels of commonly used housekeeping genes (PMID: 15627964), it is recommended that more than one loading control antibody is used while developing new assays. More information can be found here: https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/.

 

What drugs can influence beta-tubulin and organization of microtubules?

Many drugs that affect microtubule dynamics target beta-tubulin, mainly by interfering with the GTP hydrolysis (PMID: 21381049). Paclitaxel (Taxol) is used to stabilize microtubules by slowing down their depolymerization, while colchicine and vinca alkaloids (vinblastine) destabilize microtubules. They are used in research and also in the clinic as anti-cancer agents.

 

Is beta-tubulin post-translationally modified?

Yes, tubulins are subject to extensive post-translational modifications (PTMs) that affect the organization of microtubules and their dynamics. The most common modifications include polyglutamylation, polyglycylation, polyamination, glycososylation, glycation, phosphorylation, and acetylation (PMID: 24801181 and 25468068).

实验方案

Product Specific Protocols
WB protocol for Beta Tubulin antibody 66240-1-IgDownload protocol
IHC protocol for Beta Tubulin antibody 66240-1-IgDownload protocol
IF protocol for Beta Tubulin antibody 66240-1-IgDownload protocol
IP protocol for Beta Tubulin antibody 66240-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Biotechnol

Magnify is a universal molecular anchoring strategy for expansion microscopy

Authors - Aleksandra Klimas
WB

Nature

IL-27 signalling promotes adipocyte thermogenesis and energy expenditure.

Authors - Qian Wang
humanIF

Nature

ER proteins decipher the tubulin code to regulate organelle distribution

Authors - Pengli Zheng
humanWB

Science

A prometastatic splicing program regulated by SNRPA1 interactions with structured RNA elements.

Authors - Lisa Fish
WB

Adv Mater

A Physiologically Responsive Nanocomposite Hydrogel for Treatment of Head and Neck Squamous Cell Carcinoma via Proteolysis-targeting Chimeras Enhanced Immunotherapy

Authors - Yaping Wu
mouseWB

Cell Metab

Mapping the single-cell transcriptomic response of murine diabetic kidney disease to therapies.

Authors - Haojia Wu