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验证数据展示

  • IHC staining of mouse testis using 30112-1-AP

    IHC staining of mouse testis using 30112-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30112-1-AP (TTC21B antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 30112-1-AP

    WB analysis using 30112-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30112-1-AP (TTC21B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

TTC21B Polyclonal antibody

TTC21B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30112-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, ELISA

别名

FLJ11457, IFT139, KIAA1992, Nbla10696, THM1, TPR repeat protein 21B, TTC21B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, K-562 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30112-1-AP targets TTC21B in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse
免疫原

CatNo: Ag32456

Product name: Recombinant human TTC21B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 149-462 aa of NM_024753

Sequence: AWLDITRGKEPYTKKALKYFEEGLQDGNDTFALLGKAQCLEMRQNYSGALETVNQIIVNFPSFLPAFVKKMKLQLALQDWDQTVETAQRLLLQDSQNVEALRMQALYYVCREGDIEKASTKLENLGNTLDAMEPQNAQLFYNITLAFSRTCGRSQLILQKIQTLLERAFSLNPQQSEFATELGYQMILQGRVKEALKWYKTAMTLDETSVSALVGFIQCQLIEGQLQDADQQLEFLNEIQQSIGKSAELIYLHAVLAMKKNKRQEEVINLLNDVLDTHFSQLEGLPLGIQYFEKLNPDFLLEIVMEYLSFCPMQ*

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tetratricopeptide repeat domain 21B
别名 FLJ11457, IFT139, KIAA1992, Nbla10696, THM1, TPR repeat protein 21B, TTC21B
计算分子量151KD
观测分子量 55 kDa
GenBank蛋白编号NM_024753
基因名称 TTC21B
Gene ID (NCBI) 79809
RRIDAB_3086232
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z4L5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TTC21B, also known as THM1, IFT139, belongs to the TTC21 family, containing several tetratricopeptide repeat (TPR) domains. TTC21B is a component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs). TTC21B is essential for retrograde trafficking of IFT-1, IFT-B, and GPCRs (PubMed:27932497). TTC21B is localized to the cilium axoneme and may play a role in retrograde intraflagellar transport in cilia (PMID: 18327258). Mutations in TTC21B are associated with nephronophthisis 12 (NPHP12)and short-rib thoracic dysplasia 4 with or without polydactyly (SRTD4)(PMID: 21258341).

实验方案

Product Specific Protocols
IHC protocol for TTC21B antibody 30112-1-APDownload protocol
WB protocol for TTC21B antibody 30112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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