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验证数据展示

  • WB analysis using 29957-1-AP

    WB analysis using 29957-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29957-1-AP (TSR1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IF Staining of U-251 using 29957-1-AP

    IF Staining of U-251 using 29957-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using TSR1 antibody (29957-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

TSR1 Polyclonal antibody

TSR1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29957-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Pre-rRNA-processing protein TSR1 homolog, KIAA1401, FLJ10534

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, L02 cells, U-251 cells
Positive IF/ICC detected inU-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29957-1-AP targets TSR1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag31575

Product name: Recombinant human TSR1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 456-804 aa of NM_018128

Sequence: DDLYDKKVDEEAEAKMLEKYKQERLEEMFPDEVDTPRDVAARIRFQKYRGLKSFRTSPWDPKENLPQDYARIFQFQNFTNTRKSIFKEVEEKEVEGAEVGWYVTLHVSEVPVSVVECFRQGTPLIAFSLLPHEQKMSVLNMVVRRDPGNTEPVKAKEELIFHCGFRRFRASPLFSQHTAADKHKLQRFLTADMALVATVYAPITFPPASVLLFKQKSNGMHSLIATGHLMSVDPDRMVIKRVVLSGHPFKIFTKMAVVRYMFFNREDVLWFKPVELRTKWGRRGHIKEPLGTHGHMKCSFDGKLKSQDTVLMNLYKRVFPKWTYDPYVPEPVPWLKSEISSTVPQGGME

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TSR1, 20S rRNA accumulation, homolog (S. cerevisiae)
别名 Pre-rRNA-processing protein TSR1 homolog, KIAA1401, FLJ10534
计算分子量92KD
观测分子量 100 kDa
GenBank蛋白编号NM_018128
基因名称 TSR1
Gene ID (NCBI) 55720
RRIDAB_3086199
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ2NL82
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for TSR1 antibody 29957-1-APDownload protocol
WB protocol for TSR1 antibody 29957-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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