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验证数据展示

  • WB analysis using 31906-1-AP

    WB analysis using 31906-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31906-1-AP (TRNT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 31906-1-AP

    IF Staining of HepG2 using 31906-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRNT1 antibody (31906-1-AP) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

TRNT1 Polyclonal antibody

TRNT1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31906-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Mitochondrial tRNA nucleotidyl transferase, CCA-adding, EC:2.7.7.72, CGI-47, CCA-Adding Enzyme, CCA1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TRNT1 Ready-To-Use IHC Kit. TRNT1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31906-1-AP targets TRNT1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag36149

Product name: Recombinant human TRNT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 271-405 aa of BC012537

Sequence: FKVQDDVTKLDLRLKIAKEEKNLGLFIVKNRKDLIKATDSSDPLKPYQDFIIDSREPDATTRVCELLKYQGEHCLLKEMQQWSIPPFPVSGHDIRKVGISSGKEIGALLQQLREQWKKSGYQMEKDELLSYIKKT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tRNA nucleotidyl transferase, CCA-adding, 1
别名 Mitochondrial tRNA nucleotidyl transferase, CCA-adding, EC:2.7.7.72, CGI-47, CCA-Adding Enzyme, CCA1
计算分子量50 kDa
观测分子量45 kDa
GenBank蛋白编号BC012537
基因名称 TRNT1
Gene ID (NCBI) 51095
RRIDAB_3670139
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ96Q11
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

tRNA nucleotidyl transferase 1 (TRNT1, also known as CCA1, RPEM, SIFD, MtCCA, and CGI-47 ) is a cytosine-cytosine-adenine (CCA) - adding enzyme which belongs to the tRNA nucleotidyltransferase/poly (A) polymerase family. It can catalyze the addition of the conserved nucleotide triplet CCA to the 3' terminus of tRNA molecules (PMID: 27370603). Mutations in TRNT1 would cause congenital sideroblastic anemia with immunodeficiency, fevers, and developmental delay (SIFD) (PMID: 25193871).

实验方案

Product Specific Protocols
IF protocol for TRNT1 antibody 31906-1-APDownload protocol
WB protocol for TRNT1 antibody 31906-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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