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验证数据展示

  • WB analysis using 85425-2-RR

    WB analysis using 85425-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85425-2-RR (DcR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 85425-2-RR

    WB analysis using 85425-2-RR

    Human kidney lysate was subjected to SDS PAGE followed by western blot with 85425-2-RR (DcR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 85425-2-RR

    IHC staining of mouse liver using 85425-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85425-2-RR (DcR2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 85425-2-RR

    IHC staining of mouse liver using 85425-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85425-2-RR (DcR2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DcR2 Recombinant antibody

DcR2 Recombinant Antibody for WB, IHC, ELISA
Cat No. 85425-2-RR

产品说明书

CloneNo. 242916C11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CD264, Decoy receptor 2, TNF-related apoptosis-inducing ligand receptor 4, TNFRSF10D, TRAIL R4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman kidney tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85425-2-RR targets DcR2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DcR2 fusion protein Eg3487 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain
别名 CD264, Decoy receptor 2, TNF-related apoptosis-inducing ligand receptor 4, TNFRSF10D, TRAIL R4
计算分子量42 kDa
观测分子量50-55 kDa
GenBank蛋白编号NM_003840.5
基因名称 DcR2
Gene ID (NCBI) 8793
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UBN6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Decoy receptor 2 (DcR2) is a member of the tumor necrosis factor receptor (TNFR) superfamily. It functions as a decoy receptor for TNF-related apoptosis-inducing ligand (TRAIL), thereby inhibiting TRAIL-mediated apoptosis (PMID: 15538968; 9537512). It has been considered a tentative marker of cellular senescence (PMID: 16079833; 22751116). Additionally, DCR2 has been implicated in the regulation of renal fibrosis and cell senescence, contributing to the pathogenesis of diabetic nephropathy (PMID: 35661704).

实验方案

Product Specific Protocols
WB protocol for DcR2 antibody 85425-2-RRDownload protocol
IHC protocol for DcR2 antibody 85425-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols