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验证数据展示

  • WB analysis using 32528-1-AP

    WB analysis using 32528-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32528-1-AP (TNFRSF11A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

RANK/TNFRSF11A Polyclonal antibody

RANK/TNFRSF11A Polyclonal Antibody for WB, ELISA
Cat No. 32528-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, ELISA

别名

TNFRSF11A, TNF receptor superfamily member 11A , Receptor activator of NF-KB, RANK, Osteoclast differentiation factor receptor (ODFR)

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inC2C12 cells, rat liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32528-1-AP targets RANK/TNFRSF11A in WB, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Tumor necrosis factor receptor superfamily, member 11a
别名 TNFRSF11A, TNF receptor superfamily member 11A , Receptor activator of NF-KB, RANK, Osteoclast differentiation factor receptor (ODFR)
计算分子量68 kDa
观测分子量70 kDa
GenBank蛋白编号NM_001271235.1
基因名称 Tnfrsf11a
Gene ID (NCBI) 498206
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDF1M8Z6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tumor necrosis factor receptor superfamily member 11A (TNFRSF11A, also known as RANK, CD265, and ODFR) is the signaling receptor essential for osteoclast differentiation factor-mediated osteoclastogenesis and is involved in the regulation of interactions between T-cells and dendritic cells (PMID: 9878548). The RANK/RANKL/OPG system is a key regulator in bone resorption, and its dysregulation can lead to pathological bone loss (PMID: 37893470).

实验方案

Product Specific Protocols
WB protocol for RANK/TNFRSF11A antibody 32528-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols