验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells |
| Positive IHC detected in | rat kidney tissue, human cervical cancer tissue, human ovary tumor tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13321-1-AP targets TSG-6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | TSG-6 fusion protein Ag3885 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tumor necrosis factor, alpha-induced protein 6 |
| 别名 | TNFAIP6, EC:3.1.1.-, Hyaluronate binding protein, Hyaluronate-binding protein, TNF alpha induced protein 6 |
| 计算分子量 | 277 aa, 31 kDa |
| 观测分子量 | 39 kDa |
| GenBank蛋白编号 | BC030205 |
| 基因名称 | TNFAIP6 |
| Gene ID (NCBI) | 7130 |
| RRID | AB_2204641 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P98066 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TSG-6 was first discovered by Lee when screening the cDNA expression library of human fibroblasts interfered by TNF-α. TSG-6 is expressed in normal fibroblasts, peripheral blood mononuclear cells, synovial cells and chondrocytes. It is constitutively expressed in tissue cells with immune barrier, high basal metabolism level and differentiation potential (such as mesenchymal stem cells), but it is usually not expressed in normal tissue cells, but can be induced by inflammatory mediators. TSG-6 has the functions of anti-inflammation, extracellular matrix remodeling and local microenvironment protection. It plays an important role in the repair of joint inflammation by binding with various ligands, including glycosaminoglycan, proteoglycan core protein and other matrix components, and directly binding with various chemokines and bone morphogenetic proteins. TSG-6 has shown anti-inflammatory effects in many diseases, including skin wound closure, pancreatitis, conjunctivitis, neuritis, intestinal ischemia-reperfusion injury, liver fibrosis, acute myocardial infarction and so on. It is the secreted protein product of tumor necrosis factor-stimulating gene-6 (TSG-6) with a molecular mass of about 35-38 kDa.(PMID: 34707499)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TSG-6 antibody 13321-1-AP | Download protocol |
| IHC protocol for TSG-6 antibody 13321-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
JACC Basic Transl Sci Atheroprotective Effects of Tumor Necrosis Factor-Stimulated Gene-6. | ||
Front Pharmacol TSG-6 Inhibits Oxidative Stress and Induces M2 Polarization of Hepatic Macrophages in Mice With Alcoholic Hepatitis via Suppression of STAT3 Activation. | ||
Neurochem Res Tumor Necrosis Factor-stimulated Gene-6 (TSG-6) Secreted by BMSCs Regulates Activated Astrocytes by Inhibiting NF-κB Signaling Pathway to Ameliorate Blood Brain Barrier Damage After Intracerebral Hemorrhage. | ||
Contrast Media Mol Imaging TNFAIP6 Promotes Gastric Carcinoma Cell Invasion via Upregulating PTX3 and Activating the Wnt/β-Catenin Signaling Pathway
| ||
Neural Regen Res Tumor necrosis factor-stimulated gene-6 ameliorates early brain injury after subarachnoid hemorrhage by suppressing NLRC4 inflammasome-mediated astrocyte pyroptosis | ||
Front Immunol Neutrophil infiltration associated genes on the prognosis and tumor immune microenvironment of lung adenocarcinoma |