TNF Alpha Polyclonal antibody
TNF Alpha Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | PMA, LPS and Brefeldin A treated THP-1 cells |
Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 11 publications below |
IHC | See 9 publications below |
产品信息
26405-1-AP targets TNF Alpha in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | IHC, WB, ELISA |
Cited Applications | WB, IHC |
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat |
Immunogen | TNF Alpha fusion protein Ag24020 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | tumor necrosis factor (TNF superfamily, member 2) |
Synonyms | Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-a, TNF-alpha, TNFSF2, TNFα, TNF-α, Tumor necrosis factor |
Calculated Molecular Weight | 233 aa, 26 kDa |
Observed Molecular Weight | 26 kDa |
GenBank Accession Number | BC028148 |
Gene Symbol | TNF |
Gene ID (NCBI) | 7124 |
ENSEMBL Gene ID | ENSG00000232810 |
RRID | AB_2918102 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P01375 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for TNF Alpha antibody 26405-1-AP | Download protocol |
IHC protocol for TNF Alpha antibody 26405-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Redox Biol Ethyl carbamate triggers ferroptosis in liver through inhibiting GSH synthesis and suppressing Nrf2 activation. | ||
Cell Prolif Dental pulp stem cells-based therapy for the oviduct injury via immunomodulation and angiogenesis in vivo. | ||
Neurochem Res BMP7 Attenuates Neuroinflammation after Spinal Cord Injury by Suppressing the Microglia Activation and Inducing Microglial Polarization Via the STAT3 Pathway | ||
J Alzheimers Dis Metabolic Reprogramming of Microglia Enhances Proinflammatory Cytokine Release through EphA2/p38 MAPK Pathway in Alzheimer's Disease | ||
Naunyn Schmiedebergs Arch Pharmacol Songorine ameliorates LPS-induced sepsis cardiomyopathy by Wnt/β-catenin signaling pathway-mediated mitochondrial biosynthesis | ||
Biochem Biophys Res Commun Btk knockout attenuates the liver inflammation in STZ-induced diabetic mice by suppressing NLRP3 inflammasome activation |