验证数据展示
产品信息
27489-1-PBS targets TMX1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, Indirect ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag26595 Product name: Recombinant human TMX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 202-280 aa of BC036460 Sequence: VADCLCPSKRRRPQPYPYPSKKLLSESAQPLKKVEEEQEADEEDVSEEEAESKEGTNKDFPQNAIRQRSLGPSLATDKS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | thioredoxin-related transmembrane protein 1 |
| 别名 | Thioredoxin-related transmembrane protein 1, Thioredoxin domain-containing protein 1, PSEC0085, Protein disulfide-isomerase TMX1, PDIA11 |
| 计算分子量 | 32 kDa |
| 观测分子量 | 31 kDa |
| GenBank蛋白编号 | BC036460 |
| 基因名称 | TMX1 |
| Gene ID (NCBI) | 81542 |
| RRID | AB_2880886 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H3N1 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
TMX1, one of the few transmembrane members of the family, is the first vascular thiol isomerase with antithrombotic function. It forms functional complexes with the ER lectin calnexin and preferentially intervenes during maturation of cysteine-containing, membrane-associated proteins while ignoring the same cysteine-containing ectodomains if not anchored at the ER membrane. As such, TMX1 is the first example of a topology-specific client protein redox catalyst in living cells(PMID: 30655304). Covalent modification with AMS increases the molecular mass of originally oxidized species by ~0.5 kDa per thiol group, which allows enhanced separation from the reduced form in electrophoresis(PMID: 29123984). TMX1 has 2 bands produces by redox with the MW of 30-32 kDa.
