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验证数据展示

  • WB analysis using 30639-1-AP

    WB analysis using 30639-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30639-1-AP (TMEM33 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

TMEM33 Polyclonal antibody

TMEM33 Polyclonal Antibody for WB, ELISA
Cat No. 30639-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, ELISA

别名

Transmembrane protein 33, SHINC-3, SHINC3, Protein DB83, DB83

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30639-1-AP targets TMEM33 in WB, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 mouse, rat
免疫原 TMEM33 fusion protein Ag32368 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 33
别名 Transmembrane protein 33, SHINC-3, SHINC3, Protein DB83, DB83
计算分子量 28 kDa
观测分子量27-30 kDa
GenBank蛋白编号BC000948
基因名称 TMEM33
Gene ID (NCBI) 55161
RRIDAB_3669743
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP57088
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM33 (transmembrane protein 33), also known as DB83. It is expected to be located in endoplasmic reticulum membrane and nucleus envelope. The protein is widely expressed in prostate cancer and several cancer cell lines (at protein level). And expressed at higher levels in endocrine-resistant breast cancer cells as compared to endocrine-sensitive breast cancer cells. It is also expressed at higher levels in early recurrence breast cancer tissues as compared to non-recurrent breast tumors. The calculated molecular weight of TMEM33 is 28 kDa. It is suggest that TMEM33 has a potency to suppress the membrane-shaping activity of reticulons, thereby regulating the tubular structure of ER (PMID: 25612671).

实验方案

Product Specific Protocols
WB protocol for TMEM33 antibody 30639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols