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验证数据展示

  • WB analysis using 24361-1-AP (same clone as 24361-1-PBS)

    WB analysis using 24361-1-AP (same clone as 24361-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24361-1-AP (TMEM25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24361-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 24361-1-AP (same clone as 24361-1-PBS)

    IHC staining of human liver using 24361-1-AP (same clone as 24361-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24361-1-AP (TMEM25 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24361-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TMEM25 antibody included
  • Featured Product
  • KD/KO验证

TMEM25 Polyclonal antibody, PBS Only

TMEM25 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24361-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TMEM25 Ready-To-Use IHC Kit. TMEM25 primary antibody included.

产品信息

24361-1-PBS targets TMEM25 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag19964

Product name: Recombinant human TMEM25 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 28-323 aa of BC051841

Sequence: LEPQIDGQTWAERALRENERHAFTCRVAGGPGTPRLAWYLDGQLQEASTSRLLSVGGEAFSGGTSTFTVTAHRAQHELNCSLQDPRSGRSANASVILNVQFKPEIAQVGAKYQEAQGPGLLVVLFALVRANPPANVTWIDQDGPVTVNTSDFLVLDAQNYPWLTNHTVQLQLRSLAHNLSVVATNDVGVTSASLPAPGPSRHPSLISSDSNNLKLNNVRLPRENMSLPSNLQLNDLTPDSRAVKPADRQMAQNNSRPELLDPEPGGLLTSRGFIRLPVLGYIYRVSSVSSDEIWL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 25
别名
计算分子量 366 aa, 39 kDa
观测分子量 40 kDa
GenBank蛋白编号BC051841
基因名称 TMEM25
Gene ID (NCBI) 84866
RRIDAB_2879507
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86YD3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TMEM25 is a novel member of the immunoglobulin superfamily. BC042896 and AY358919 cDNAs are derived from human TMEM25 gene. TMEM25 isoform 1 (BC042896), consisting of exons 1-9, encodes a 366-aa transmembrane protein. TMEM25 isoform 2 (AY358919), consisting of exons 1-4 and 6-9, encodes a 322-aa secreted protein (PMID:15254712). Human TMEM25 gene are found to encode transmembrane-type as well as secreted-type proteins due to alternative splicing of exon-skipping type. TMEM25 mRNA is expressed in brain, including cerebellar cortex and hippocampus, as well as in neuroblastoma, brain tumors, and gastric cancer. TMEM25 is a target of pharmacogenomics in the field of oncology and regenerative medicine. TMEM25 mRNAs may be useful members of a panel of favorable prognostic and predictive markers for breast cancer( PMID:19776672).