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验证数据展示

  • WB analysis of HepG2 using 68172-1-Ig

    WB analysis of HepG2 using 68172-1-Ig

    WB result of TMEM175 antibody (68172-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM175 transfected HepG2 cells.

  • WB analysis using 68172-1-Ig

    WB analysis using 68172-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68172-1-Ig (TMEM175 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IP experiment of mouse brain using 68172-1-Ig

    IP experiment of mouse brain using 68172-1-Ig

    IP result of anti-TMEM175 (IP:68172-1-Ig, 4ug; Detection:68172-1-Ig 1:4000) with mouse brain tissue lysate 1840 ug.

  • Featured Product
  • KD/KO验证

TMEM175 Monoclonal antibody

TMEM175 Monoclonal Antibody for WB, IP, ELISA
Cat No. 68172-1-Ig

产品说明书

CloneNo. 1E7E1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IP, ELISA

别名

Transmembrane protein 175, hTMEM175, 1E7E1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, pig liver tissue, rabbit liver tissue, HepG2 cells, mouse brain tissue, rat liver tissue, HeLa cells, HEK-293 cells, Jurkat cells
Positive IP detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68172-1-Ig targets TMEM175 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 TMEM175 fusion protein Ag13890 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 transmembrane protein 175
别名 Transmembrane protein 175, hTMEM175, 1E7E1
计算分子量 504 aa, 56 kDa
观测分子量55-60 kDa
GenBank蛋白编号BC005158
基因名称 TMEM175
Gene ID (NCBI) 84286
RRIDAB_2923687
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BSA9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM175 has two repeats of 6-transmembrane-spanning segments and has no GYG K+ channel sequence signature-containing, pore-forming P loop. Lysosomes lacking TMEM175 exhibit no K+conductance, have a markedly depolarized ΔΨ and little sensitivity to changes in [K+], and have compromised luminal pH stability and abnormal fusion with autophagosomes during autophagy. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability.

实验方案

Product Specific Protocols
WB protocol for TMEM175 antibody 68172-1-IgDownload protocol
IP protocol for TMEM175 antibody 68172-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols