- Featured Product
- KD/KO Validated
TMEM175 Monoclonal antibody
TMEM175 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, Pig, Rabbit
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1E7E1
验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, pig liver tissue, rabbit liver tissue, HepG2 cells, rat liver tissue, HeLa cells, HEK-293 cells, Jurkat cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68172-1-Ig targets TMEM175 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.
| Tested Applications | WB, ELISA |
| Tested Reactivity | Human, Mouse, Rat, Pig, Rabbit |
| Immunogen | TMEM175 fusion protein Ag13890 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | transmembrane protein 175 |
| Synonyms | TMEM175, transmembrane protein 175 |
| Calculated Molecular Weight | 504 aa, 56 kDa |
| Observed Molecular Weight | 55-60 kDa |
| GenBank Accession Number | BC005158 |
| Gene Symbol | TMEM175 |
| Gene ID (NCBI) | 84286 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9BSA9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TMEM175 has two repeats of 6-transmembrane-spanning segments and has no GYG K+ channel sequence signature-containing, pore-forming P loop. Lysosomes lacking TMEM175 exhibit no K+conductance, have a markedly depolarized ΔΨ and little sensitivity to changes in [K+], and have compromised luminal pH stability and abnormal fusion with autophagosomes during autophagy. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TMEM175 antibody 68172-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
