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验证数据展示

  • WB analysis using 80458-2-RR (same clone as 80458-2-PBS)

    WB analysis using 80458-2-RR (same clone as 80458-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80458-2-RR (Tgfb1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80458-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 80458-2-RR (same clone as 80458-2-PBS)

    IHC staining of mouse spleen using 80458-2-RR (same clone as 80458-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 80458-2-RR (TGF beta 1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80458-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 80458-2-RR (same clone as 80458-2-PBS)

    IHC staining of mouse spleen using 80458-2-RR (same clone as 80458-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 80458-2-RR (TGF beta 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80458-2-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 80458-2-RR (same clone as 80458-2-PBS)

    IF Staining of NIH/3T3 using 80458-2-RR (same clone as 80458-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using TGF beta 1 antibody (80458-2-RR, Clone: 250513C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80458-2-PBS in a different storage buffer formulation.

Mouse TGF beta 1 Recombinant monoclonal antibody, PBS Only

TGF beta 1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 80458-2-PBS

产品说明书

CloneNo. 250513C9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

Tgfb1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, Latency-associated peptide, TGF beta1, Tgfb

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

80458-2-PBS targets TGF beta 1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Eg3734

Product name: Recombinant mouse Tgfb1 protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 30-390 aa of NM-011577

Sequence: LSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESADPEPEPEADYYAKEVTRVLMVDRNNAIYEKTKDISHSIYMFFNTSDIREAVPEPPLLSRAELRLQRLKSSVEQHVELYQKYSNNSWRYLGNRLLTPTDTPEWLSFDVTGVVRQWLNQGDGIQGFRFSAHCSCDSKDNKLHVEINGISPKRRGDLGTIHDMNRPFLLLMATPLERAQHLHSSRHRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASASPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transforming growth factor, beta 1
别名 Tgfb1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, Latency-associated peptide, TGF beta1, Tgfb
观测分子量48 kDa
GenBank蛋白编号NM-011577
基因名称 TGF beta 1
Gene ID (NCBI) 21803
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP04202
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TGF beta 1 is a potent cytokine capable of regulating multiple cellular processes including cell proliferation, differentiation, wound healing, and immune response. TGF beta 1 is identified in various immune cells and its activity varies in different cell types or cells at different developmental stages1. TGF beta 1 functions by binding to the TGFβ1 receptors, which subsequently activate the canonical SMAD pathway or non-canonical signaling cascades, such as the mitogen-activated protein kinase (MAPK) pathway, phosphatidylinositol-3 kinase and AKT (PI3K-AKT), and Rho family of GTPases (Rho GTPase). What is special about TGF beta 1 is its context-dependent nature that is particularly true for tumors. Full-length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB (12, 25, 45 to 65 kDa).

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