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验证数据展示

  • IHC staining of mouse testis using 20858-1-AP

    IHC staining of mouse testis using 20858-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20858-1-AP (TCERG1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 20858-1-AP

    IHC staining of mouse testis using 20858-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20858-1-AP (TCERG1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 20858-1-AP

    IF Staining of mouse testis using 20858-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using TCERG1L antibody (20858-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

TCERG1L Polyclonal antibody

TCERG1L Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 20858-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF-P, ELISA

别名

Transcription elongation regulator 1-like protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20858-1-AP targets TCERG1L in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag14828

Product name: Recombinant human TCERG1L protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 337-545 aa of BC042951

Sequence: DLNRIIEDPPHKRKLEAPATDNSDGSSSEDNREDQDVKTKRNRTEGCGSPKPEEAKREDKGTRTPPPQILLPLEERVTHFRDMLLERGVSAFSTWEKELHKIVFDPRYLLLNSEERKQIFEQFVKTRIKEEYKEKKSKLLLAKEEFKKLLEKSKVSPRTTFKEFAEKYGRDQRFRLVQKRKDQEHFFNKFILILKKRDKENRLRLRKMR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transcription elongation regulator 1-like
别名 Transcription elongation regulator 1-like protein
计算分子量 586 aa, 66 kDa
GenBank蛋白编号BC042951
基因名称 TCERG1L
Gene ID (NCBI) 256536
RRIDAB_3085624
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5VWI1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TCERG1L, Transcription elongation regulator 1-like protein, is associated with plasma adiponectin, and plasma adiponectin is a key modulator of obesity, inflammation, IR and diabetes (PMID:22791750). Gene silencing of FBN2 and TCERG1L is associated with promoter DNA hypermethylation in CRC tumors and may be biomarkers for the early detection of CRC (PMID:22238052). Stra8 and TCERG1L play a role in retinoic acid induced spermatogonial differentiation in mouse (PMID:3395975).

实验方案

Product Specific Protocols
IF protocol for TCERG1L antibody 20858-1-APDownload protocol
IHC protocol for TCERG1L antibody 20858-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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