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验证数据展示

  • WB analysis using 68577-1-Ig

    WB analysis using 68577-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68577-1-Ig (TAPBPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • FC experiment of A431 using 68577-1-Ig

    FC experiment of A431 using 68577-1-Ig

    1x10^6 A431 cells were intracellularly stained with 0.4 ug TAPBPL Monoclonal antibody (68577-1-Ig, Clone: 1D12C2) and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002) (red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

TAPBPL Monoclonal antibody

TAPBPL Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 68577-1-Ig

产品说明书

CloneNo. 1D12C2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

Tapasin like, TAPASIN R, Tapasin-like, TAPASIN-R, Tapasin-related protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSCaBER cells, MCF-7 cells, HT-1376 cells, A431 cells, U-87 MG cells, Jurkat cells, human placenta tissue
Positive FC (Intra) detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68577-1-Ig targets TAPBPL in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14371

Product name: Recombinant human TAPBPL protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 24-400 aa of BC015017

Sequence: HPAEGQWRAVDVVLDCFLVKDGAHRGALASSEDRARASLVLKQVPVLDDGSLEDFTDFQGGTLAQDDPPIIFEASVDLVQIPQAEALLHADCSGKEVTCEISRYFLQMTETTVKTAAWFMANVQVSGGGPSISLVMKTPRVAKNEVLWHPTLNLPLSPQGTVRTAVEFQVMTQTQSLSFLLGSSASLDCGFSMAPGLDLISVEWRLQHKGRGQLVYSWTAGQGQAVRKGATLEPAQLGMARDASLTLPGLTIQDEGTYICQITTSLYRAQQIIQLNIQASPKVRLSLANEALLPTLICDIAGYYPLDVVVTWTREELGGSPAQVSGASFSSLRQSVAGTYSISSSLTAEPGSAGATYTCQVTHISLEEPLGASTQVV

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 TAP binding protein-like
别名 Tapasin like, TAPASIN R, Tapasin-like, TAPASIN-R, Tapasin-related protein
计算分子量 468 aa, 50 kDa
观测分子量55 kDa
GenBank蛋白编号BC015017
基因名称 TAPBPL
Gene ID (NCBI) 55080
RRIDAB_3085277
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9BX59
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TAPBPL, also named as TAPBP-R and TAPASIN-R, is a member of the variable-constant Ig superfamily that links MHC class I molecules to the transporter associated with antigen processing in the endoplasmic reticulum.

实验方案

Product Specific Protocols
FC protocol for TAPBPL antibody 68577-1-IgDownload protocol
WB protocol for TAPBPL antibody 68577-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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