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Sodium butanoate

CAS号

156-54-7

分子式

C4H7NaO2

主要靶点

Histamine Receptor

仅限科研使用

Cat No : CM00503

Print datasheet

Synonyms

Butanoic acid sodium|Butanoic acid sodium salt|HistamineReceptor|Histamine Receptor|H1 receptor|Sodium Butanoate|Sodium butanoate|Sodium Butyrate|丁酸钠

验证数据展示

  • CAS No.: 156-54-7

    Sodium butanoate
    CAS#: 156-54-7

产品信息

CAS号 156-54-7
分子式 C4H7NaO2
主要靶点 Histamine Receptor
主要通路 神经科学|免疫与炎症|G 蛋白偶联受体
分子量 110.09
纯度 99.67%， 此纯度可做参考，具体纯度与批次有关系，可咨询客服
储存条件 keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
别名 Butanoic acid sodium|Butanoic acid sodium salt|HistamineReceptor|Histamine Receptor|H1 receptor|Sodium Butanoate|Sodium butanoate|Sodium Butyrate|丁酸钠

体内活性

方法：为研究对亨廷顿舞蹈症 (HD) 的作用，将 Sodium butanoate (100-10000 mg/kg) 腹腔注射给 R6/2 小鼠，每天一次，持续至死亡。 结果：在 R6/2 转基因 HD 小鼠模型中，使用 Sodium butanoate 调节转录的药理学治疗以剂量依赖性方式显著延长了生存期，改善了体重和运动性能，并延缓了神经病理学后遗症。[3]

体外活性

方法：NPC 细胞系 5-8F 和 6-10B 用 Sodium butanoate (1-10 mM) 处理 24-72 h，使用 MTT assay 检测细胞活力。 结果：在低浓度 (1 mM) 和短暴露时间 (24 h) 下，空白组和 Sodium butanoate 处理组之间的细胞活力没有显著差异。然而，Sodium butanoateu 对 5-8F 和 6-10B 细胞的细胞毒性随着浓度的升高和暴露时间的延长而增加。[1] 方法：人胶质母细胞瘤细胞 A172 用 Sodium butanoate (2 mM) 处理 6-48 h，使用 Western Blot 检测靶点蛋白表达水平。 结果：用 Sodium butanoate 处理的 A172 细胞表现出 p21、p27 和 p53 水平的升高，并且这种表达的增加是时间依赖性的。[2]

溶解度

H2O:100 mg/mL (908.35 mM);DMSO:1.1 mg/mL (10 mM)

细胞实验

Cells are seeded at a density of 2,000 cells/well in 96-well plates with 200 μL culture medium containing Sodium Butyrate at different concentrations. Then, the cells are consecutively cultured for 72 h. Every 24 h, 20 μL 5 mg/mL MTT solution is added into the corresponding well, and the cells are cultured for another 4 h. Then, the solution is replaced with 150 μL dimethylsulfoxide, followed by gentle agitation of the plates for 15 min at room temperature. Finally, the absorbance at 492 nm is measured to represent the cell viability.

参考文献

1.Huang W, et al. Inhibition of store-operated Ca2+ entry counteracts the apoptosis of nasopharyngeal carcinoma cells induced by sodium butyrate. Oncol Lett. 2017 Feb;13(2):921-929.
2.Qiu Y, et al. Effect of sodium butyrate on cell proliferation and cell cycle in porcine intestinal epithelial (IPEC-J2) cells. In Vitro Cell Dev Biol Anim. 2017 Jan 27
3.Nakagawa H, et al. Sodium butyrate induces senescence and inhibits the invasiveness of glioblastoma cells. Oncol Lett. 2018 Feb;15(2):1495-1502.
4.Ferrante RJ, et al. Histone deacetylase inhibition by sodium butyrate chemotherapy ameliorates the neurodegenerative phenotype in Huntington's disease mice. J Neurosci. 2003 Oct 15;23(28):9418-27.
5.Davie JR, et al. J Nutr, 2003, 133(7 Suppl), 2485S-2493S.
6.Ferrante RJ, et al. J Neurosci, 2003, 23(28), 9418-9427.
7.Jung HY, et al. Sirtuin-2 inhibition affects hippocampal functions and sodium butyrate ameliorates the reduction in novel object memory, cell proliferation, and neuroblast differentiation. Lab Anim Res. 2016 Dec;32(4):224-230
8.Huang W, et al. Inhibition of store-operated Ca2+ entry counteracts the apoptosis of nasopharyngeal carcinoma cells induced by sodium butyrate. Oncol Lett. 2017 Feb;13(2):921-929
9.Wang P, et al. Sodium butyrate triggers a functional elongation of microglial process via Akt-small RhoGTPase activation and HDACs inhibition. Neurobiol Dis. 2017 Dec 14;111:12-25.
10.Jaworska J, et al. The potential neuroprotective role of a histone deacetylase inhibitor, sodium butyrate, after neonatal hypoxia-ischemia. J Neuroinflammation. 2017 Feb 10;14(1):34

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