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验证数据展示

  • WB analysis using 83202-3-RR

    WB analysis using 83202-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83202-3-RR (Sestrin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293 using 83202-3-RR

    FC experiment of HEK-293 using 83202-3-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Sestrin 2 Recombinant antibody (83202-3-RR, Clone:240034H3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83202-3-RR

    Affinity and Kinetic Characterization of 83202-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83202-3-RR against Human Sestrin 2 were performed. The affinity constant is below 1 pM.

Sestrin 2 Recombinant antibody

Sestrin 2 Uni-rAbTM Recombinant Antibody for WB, ELISA, FC (Intra)
Cat No. 83202-3-RR

产品说明书

CloneNo. 240034H3

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA, FC (Intra)

别名

SESN2, Sestrin2, SESN 2, SES2, Hypoxia-induced gene

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive FC (Intra) detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83202-3-RR targets Sestrin 2 in WB, ELISA, FC (Intra) applications and shows reactivity with Human samples.

经测试应用 WB, ELISA, FC (Intra) Application Description
经测试反应性 Human
免疫原 Sestrin 2 fusion protein Ag1247 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sestrin 2
别名 SESN2, Sestrin2, SESN 2, SES2, Hypoxia-induced gene
计算分子量 54 kDa
观测分子量54 kDa
GenBank蛋白编号BC013304
基因名称 SESN2/Sestrin 2
Gene ID (NCBI) 83667
RRIDAB_3670890
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP58004
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SESN is a family of highly conserved, stress-inducible genes that can defend the cell against oxidative damage and oncogenic signaling. SESN2 ,which belongs to the SESN family, has been implicated as a tumor suppressor that can inhibit angiogenesis and promote autophagy, underscoring the importance of elucidating the molecular mechanism by which SESN2 regulates pathways of metabolism and survival.(PMID:22363791).

实验方案

Product Specific Protocols
WB protocol for Sestrin 2 antibody 83202-3-RRDownload protocol
FC protocol for Sestrin 2 antibody 83202-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols