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验证数据展示

  • WB analysis using 14923-1-AP (same clone as 14923-1-PBS)

    WB analysis using 14923-1-AP (same clone as 14923-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14923-1-AP (SUCLG1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14923-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse kidney using 14923-1-AP (same clone as 14923-1-PBS)

    IP experiment of mouse kidney using 14923-1-AP (same clone as 14923-1-PBS)

    IP result of anti-SUCLG1 (IP:14923-1-AP, 4ug; Detection:14923-1-AP 1:500) with mouse kidney tissue lysate 2800 ug. This data was developed using the same antibody clone with 14923-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 14923-1-AP (same clone as 14923-1-PBS)

    IHC staining of mouse kidney using 14923-1-AP (same clone as 14923-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14923-1-AP (SUCLG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14923-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 14923-1-AP (same clone as 14923-1-PBS)

    IHC staining of mouse kidney using 14923-1-AP (same clone as 14923-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14923-1-AP (SUCLG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14923-1-PBS in a different storage buffer formulation.

SUCLG1 Polyclonal antibody, PBS Only

SUCLG1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 14923-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

14923-1-PBS targets SUCLG1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6481

Product name: Recombinant human SUCLG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-333 aa of BC000504

Sequence: MVSGSSGLAAARLLSRSFLLPQNGIRHCSYTASRQHLYVDKNTKIICQGFTGKQGTFHSQQALEYGTKLVGGTTPGKGGQTHLGLPVFNTVKEAKEQTGATASVIYVPPPFAAAAINEAIEAEIPLVVCITEGIPQQDMVRVKHKLLRQEKTRLIGPNCPGVINPGECKIGIMPGHIHKKGRIGIVSRSGTLTYEAVHQTTQVGLGQSLCVGIGGDPFNGTDFIDCLEIFLNDSATEGIILIGEIGGNAEENAAEFLKQHNSGPNSKPVVSFIAGLTAPPGRRMGHAGAIIAGGKGGAKEKISALQSAGVVVSMSPAQLGTTIYKEFEKRKML

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 succinate-CoA ligase, alpha subunit
别名
计算分子量 35 kDa
观测分子量 35 kDa
GenBank蛋白编号BC000504
基因名称 SUCLG1
Gene ID (NCBI) 8802
RRIDAB_2197177
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP53597
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SUCLG1(succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial) is also named as SCS-alpha and belongs to the succinate/malate CoA ligase alpha subunit family. The SUCLG1 gene encodes a 35 kDa protein with a 40 amino acids transit peptide. This enzyme catalyzes the ATP- or GTP-dependent ligation of succinate and CoA to form succinyl-CoA. Defects in SUCLG1 are the cause of mitochondrial DNA depletion syndrome type 9 (MTDPS9).

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