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验证数据展示

  • IHC staining of human lymphoma using 25471-1-AP (same clone as 25471-1-PBS)

    IHC staining of human lymphoma using 25471-1-AP (same clone as 25471-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25471-1-AP (STK10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25471-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 25471-1-AP (same clone as 25471-1-PBS)

    IHC staining of human lymphoma using 25471-1-AP (same clone as 25471-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25471-1-AP (STK10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25471-1-PBS in a different storage buffer formulation.

  • WB analysis of SKOV-3 using 25471-1-AP (same clone as 25471-1-PBS)

    WB analysis of SKOV-3 using 25471-1-AP (same clone as 25471-1-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25471-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

STK10 Polyclonal antibody, PBS Only

STK10 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25471-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

EC:2.7.11.1, LOK, Lymphocyte oriented kinase, Lymphocyte-oriented kinase, PRO2729

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

25471-1-PBS targets STK10 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag22169

Product name: Recombinant human STK10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 369-519 aa of BC070077

Sequence: SQSQDSVNEPCSQPSGDRSLQTTSPPVVAPGNENGLAVPVPLRKSRPVSMDARIQVAQEKQVAEQGGDLSPAANRSQKASQSRPNSSALETLGGEKLANGSLEPPAQAAPGPSKRDSDCSSLCTSESMDYGTNLSTDLSLNKEMGSLSIKD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serine/threonine kinase 10
别名 EC:2.7.11.1, LOK, Lymphocyte oriented kinase, Lymphocyte-oriented kinase, PRO2729
计算分子量 968 aa, 112 kDa
观测分子量 130 kDa
GenBank蛋白编号BC070077
基因名称 STK10
Gene ID (NCBI) 6793
RRIDAB_2880096
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94804
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Lymphocyte-oriented kinase deficiency encoded by the serine/threonine kinase 10 (STK10) gene correlates with the intracellular adhesion molecule 1 (ICAM-1)/lymphocyte function associated antigen 1 (LFA-1) complex in aspirin hypersensitivity(PMID:21905501). STK 10 is also named as LOK and expressed as a 130-kDa protein, which was detected predominantly in lymphoid organs such as spleen, thymus, and bone marrow, in contrast to other mammalian members of the STE20 family(PMID:9278426).

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