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STEAP2 Polyclonal antibody

STEAP2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 24804-1-AP

Print datasheet

Synonyms

IPCA1, Metalloreductase STEAP2, PCANAP1, PUMPCn, STAMP1, STEAP2, STMP

验证数据展示

  • IHC staining of human colon cancer using 24804-1-AP

    IHC staining of human colon cancer using 24804-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24804-1-AP (STEAP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 24804-1-AP

    IHC staining of human colon cancer using 24804-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24804-1-AP (STEAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 24804-1-AP

    WB analysis using 24804-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24804-1-AP (STEAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inDU 145 cells, HEK-293T cells, U2OS cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:800-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24804-1-AP targets STEAP2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human
Immunogen STEAP2 fusion protein Ag20293 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name six transmembrane epithelial antigen of the prostate 2
Synonyms IPCA1, Metalloreductase STEAP2, PCANAP1, PUMPCn, STAMP1, STEAP2, STMP
Calculated Molecular Weight 490 aa, 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC148824
Gene Symbol STEAP2
Gene ID (NCBI) 261729
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFT2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STEAP2, also named as PCANAP1 and STAMP1, belongs to the STEAP family. It is a metalloreductase that has the ability to reduce both Fe3+ to Fe2+ and Cu2+ to Cu1+. STEAP2 uses NAD+ as acceptor.

实验方案

Product Specific Protocols
WB protocol for STEAP2 antibody 24804-1-APDownload protocol
IHC protocol for STEAP2 antibody 24804-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols