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验证数据展示

  • WB analysis using 31732-1-AP

    WB analysis using 31732-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31732-1-AP (STAP1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 31732-1-AP

    IF Staining of A431 using 31732-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using STAP1 antibody (31732-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

STAP1 Polyclonal antibody

STAP1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31732-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

STAP-1, Docking protein BRDG1, BRDG1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRaji cells, Ramos cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31732-1-AP targets STAP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35196

Product name: Recombinant human STAP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 139-293 aa of BC014958

Sequence: HEVLEREKKRRIETEQSTSVEKEKEPTEDYVDVLNPMPACFYTVSRKEATEMLQKNPSLGNMILRPGSDSRNYSITIRQEIDIPRIKHYKVMSVGQNYTIELEKPVTLPNLFSVIDYFVKETRGNLRPFICSTDENTGQEPSMEGRSEKLKKNPH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 signal transducing adaptor family member 1
别名 STAP-1, Docking protein BRDG1, BRDG1
计算分子量 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC014958
基因名称 STAP1
Gene ID (NCBI) 26228
RRIDAB_3670102
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9ULZ2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Signal transducing adaptor protein 1 (STAP1), also known as BRDG1, contains a proline-rich region, a pleckstrin homology (PH) domain, and a region in the carboxy terminal half with similarity to the Src Homology 2 (SH2) domain. STAP1 acts downstream of the Tec tyrosine kinase and participates in a positive feedback loop by increasing the activity of Tec (PMID: 10518561).

实验方案

Product Specific Protocols
IF protocol for STAP1 antibody 31732-1-APDownload protocol
WB protocol for STAP1 antibody 31732-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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