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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis using 14015-1-AP (same clone as 14015-1-PBS)

    WB analysis using 14015-1-AP (same clone as 14015-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14015-1-AP (STAG1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14015-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 14015-1-AP (same clone as 14015-1-PBS)

    IHC staining of human stomach using 14015-1-AP (same clone as 14015-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14015-1-AP (STAG1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14015-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STAG1 antibody included

STAG1 Polyclonal antibody, PBS Only

STAG1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14015-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

His SA, Stromal antigen 1, SCC3, SA1, Cohesin subunit SA-1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy STAG1 Ready-To-Use IHC Kit. STAG1 primary antibody included.

产品信息

14015-1-PBS targets STAG1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 STAG1 fusion protein Ag5123 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 stromal antigen 1
别名 His SA, Stromal antigen 1, SCC3, SA1, Cohesin subunit SA-1
计算分子量 144 kDa
观测分子量 155 kDa
GenBank蛋白编号BC064699
基因名称 STAG1
Gene ID (NCBI) 10274
RRIDAB_2194759
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WVM7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STAG1, also named as SCC3 homolog 1, is a 1258 amino acid protein, which belongs to the SCC3 family. STAG1 interacts directly with RAD21 in cohesin complex and localizes in nucleus. STAG1 is a Component of cohesin complex, which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. The calculate molecular weight of STAG1 is 144 kDa, but modified STAG1 is about 145-150 kDa.