验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87628-1-RR targets ST5 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag34032 Product name: Recombinant human ST5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 10-344 aa of BC036655 Sequence: SITHGAGGTKAPRGTLSRSQSVSPPPVLSPPRSPIYPLSDSETSACRYPSHSSSRVLLKDRHPPAPSPQNPQDPSPDTSPPTCPFKTASFGYLDRSPSACKRDAQKESVQGAAQDVAGVAACLPLAQSTPFPGPAAGPRGVLLTRTGTRAHSLGIREKISAWEGRREASPRMSMCGEKREGSGSEWAASEGCPSLGCPSVVPSPCSSEKTFDFKGLRRMSRTFSECSYPETEEEGEALPVRDSFYRLEKRLGRSEPSAFLRGHGSRKESSAVLSRIQKIEQVLKEQPGRGLPQLPSSCYSVDRGKRNTGTLGSLEEPAGGASVSAGSRAVGVAGV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | suppression of tumorigenicity 5 |
| 别名 | DENN domain-containing protein 2B, DENND2B, HTS1 |
| 计算分子量 | 1137 aa, 126 kDa |
| 观测分子量 | 120-150 kDa |
| GenBank蛋白编号 | BC036655 |
| 基因名称 | ST5 |
| Gene ID (NCBI) | 6764 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P78524 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ST5, also known as DEN2B (DENN domain-containing protein 2B), has three isoforms with molecular weights of 126 kDa, 82 kDa, and 70 kDa, respectively. The mainstream molecular weight is 126kd. ST5 is widely expressed except in peripheral blood lymphocytes. Isoform 1 is expressed in several epithelial cells and fibroblasts (including tumorigenic cells), but not in lymphoid cell lines (at the protein level).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ST5 antibody 87628-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
