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验证数据展示

  • WB analysis of mouse ovary using 16294-1-AP

    WB analysis of mouse ovary using 16294-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16294-1-AP (SPAG7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 16294-1-AP

    IHC staining of human colon cancer using 16294-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 16294-1-AP (SPAG7 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon cancer using 16294-1-AP

    IHC staining of human colon cancer using 16294-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 16294-1-AP (SPAG7 antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis using 16294-1-AP

    WB analysis using 16294-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16294-1-AP (SPAG7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

SPAG7 Polyclonal antibody

SPAG7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16294-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ACRP, FSA 1, SPAG7, sperm associated antigen 7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse ovary tissue, mouse brain tissue, mouse skeletal muscle tissue, rat brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16294-1-AP targets SPAG7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SPAG7 fusion protein Ag9471 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sperm associated antigen 7
别名 ACRP, FSA 1, SPAG7, sperm associated antigen 7
计算分子量 227 aa, 26 kDa
观测分子量 26-31 kDa
GenBank蛋白编号BC011934
基因名称 SPAG7
Gene ID (NCBI) 9552
RRIDAB_2302478
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75391
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPAG7 encodes the sperm-associated antigen 7, which is widely expressed. The SPAG7 protein contains two nuclear localisation signals and a R3H domain. The domain is predicted to bind ssDNA or ssRNA in a sequence-specific manner. This might indicate a potential role of the protein in response to viruses or free DNAs or RNAs.

实验方案

Product Specific Protocols
WB protocol for SPAG7 antibody 16294-1-APDownload protocol
IHC protocol for SPAG7 antibody 16294-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols