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SPAG7 Polyclonal antibody

SPAG7 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16294-1-AP

Print datasheet

Synonyms

ACRP, FSA 1, SPAG7, sperm associated antigen 7

验证数据展示

  • WB analysis of mouse ovary using 16294-1-AP

    WB analysis of mouse ovary using 16294-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16294-1-AP (SPAG7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 16294-1-AP

    IHC staining of human colon cancer using 16294-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 16294-1-AP (SPAG7 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon cancer using 16294-1-AP

    IHC staining of human colon cancer using 16294-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 16294-1-AP (SPAG7 antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis using 16294-1-AP

    WB analysis using 16294-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16294-1-AP (SPAG7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse ovary tissue, mouse brain tissue, mouse skeletal muscle tissue, rat brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16294-1-AP targets SPAG7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen SPAG7 fusion protein Ag9471 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sperm associated antigen 7
Synonyms ACRP, FSA 1, SPAG7, sperm associated antigen 7
Calculated Molecular Weight 227 aa, 26 kDa
Observed Molecular Weight 26-31 kDa
GenBank Accession NumberBC011934
Gene Symbol SPAG7
Gene ID (NCBI) 9552
RRIDAB_2302478
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75391
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPAG7 encodes the sperm-associated antigen 7, which is widely expressed. The SPAG7 protein contains two nuclear localisation signals and a R3H domain. The domain is predicted to bind ssDNA or ssRNA in a sequence-specific manner. This might indicate a potential role of the protein in response to viruses or free DNAs or RNAs.

实验方案

Product Specific Protocols
WB protocol for SPAG7 antibody 16294-1-APDownload protocol
IHC protocol for SPAG7 antibody 16294-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols