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验证数据展示

  • WB analysis of mouse ovary using 16294-1-AP

    WB analysis of mouse ovary using 16294-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16294-1-AP (SPAG7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 16294-1-AP

    IHC staining of human colon cancer using 16294-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 16294-1-AP (SPAG7 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon cancer using 16294-1-AP

    IHC staining of human colon cancer using 16294-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 16294-1-AP (SPAG7 antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis using 16294-1-AP

    WB analysis using 16294-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16294-1-AP (SPAG7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

SPAG7 Polyclonal antibody

SPAG7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16294-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ACRP, FSA 1, SPAG7, sperm associated antigen 7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse ovary tissue, mouse brain tissue, mouse skeletal muscle tissue, rat brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16294-1-AP targets SPAG7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9471

Product name: Recombinant human SPAG7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-227 aa of BC011934

Sequence: MADLLGSILSSMEKPPSLGDQETRRKAREQAARLKKLQEQEKQQKVEFRKRMEKEVSDFIQDSGQIKKKFQPMNKIERSILHDVVEVAGLTSFSFGEDDDCRYVMIFKKEFAPSDEELDSYRRGEEWDPQKAEEKRKLKELAQRQEEEAAQQGPVVVSPASDYKDKYSHLIGKGAAKDAAHMLQANKTYGCVPVANKRDTRSIEEAMNEIRAKKRLRQSGEELPPTS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sperm associated antigen 7
别名 ACRP, FSA 1, SPAG7, sperm associated antigen 7
计算分子量 227 aa, 26 kDa
观测分子量 26-31 kDa
GenBank蛋白编号BC011934
基因名称 SPAG7
Gene ID (NCBI) 9552
RRIDAB_2302478
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75391
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPAG7 encodes the sperm-associated antigen 7, which is widely expressed. The SPAG7 protein contains two nuclear localisation signals and a R3H domain. The domain is predicted to bind ssDNA or ssRNA in a sequence-specific manner. This might indicate a potential role of the protein in response to viruses or free DNAs or RNAs.

实验方案

Product Specific Protocols
WB protocol for SPAG7 antibody 16294-1-APDownload protocol
IHC protocol for SPAG7 antibody 16294-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols