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验证数据展示

  • WB analysis using 12374-1-AP

    WB analysis using 12374-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12374-1-AP (SNX12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 12374-1-AP

    IF Staining of HeLa using 12374-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SNX12 antibody (12374-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

SNX12 Polyclonal antibody

SNX12 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 12374-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Sorting nexin-12

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12374-1-AP targets SNX12 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3014

Product name: Recombinant human SNX12 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-162 aa of BC020559

Sequence: MSDTAVADTRRLNSKPQDLTDAYGPPSNFLEIDIFNPQTVGVGRARFTTYEVRMRTNLPIFKLKESCVRRRYSDFEWLKNELERDSKIVVPPLPGKALKRQLPFRGDEGIFEESFIEERRQGLEQFINKIAGHPLAQNERCLHMFLQEEAIDRNYVPGKSLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sorting nexin 12
别名 Sorting nexin-12
计算分子量 172 aa, 20 kDa
观测分子量 18 kDa
GenBank蛋白编号BC020559
基因名称 SNX12
Gene ID (NCBI) 29934
RRIDAB_3085392
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UMY4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX12 (Sorting nexin 12) is localized in early endosomes and SNX12 overexpression specifically prevents the degradative pathway from early to late endosomes/lysosomes (PMID: 22719997). SNX12 may participate in Alzheimer's disease (AD) pathology by regulating the endocytosis of BACE1 and affecting β-processing of APP (PMID: 22709416).

实验方案

Product Specific Protocols
IF protocol for SNX12 antibody 12374-1-APDownload protocol
WB protocol for SNX12 antibody 12374-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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