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验证数据展示

  • WB analysis of NIH/3T3 using 12799-1-AP

    WB analysis of NIH/3T3 using 12799-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12799-1-AP (SMAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 12799-1-AP

    IHC staining of human stomach using 12799-1-AP

    Immunohistochemical analysis of paraffin-embedded normal stomach slide using 12799-1-AP (SMAP1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SMAP1 Polyclonal antibody

SMAP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12799-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Stromal membrane-associated protein 1, SMAP 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells, human adrenal gland tissue, human plasma, mouse brain tissue, rat lymph tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12799-1-AP targets SMAP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3544

Product name: Recombinant human SMAP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-287 aa of BC036123

Sequence: MATRSCREKAQKLNEQHQLILSKLLREEDNKYCADCEAKGPRWASWNIGVFICIRCAGIHRNLGVHISRVKSVNLDQWTAEQIQCMQDMGNTKARLLYEANLPENFRRPQTDQAVEFFIRDKYEKKKYYDKNAIAITNISSSDAPLQPLVSSPSLQAAVDKNKLEKEKEKKKEEKKREKEPEKPAKPLTAEKLQKKDQQLEPKKSTSPKKAAEPTVDLLGLDGPAVAPVTNGNTTVPPLNDDLDIFGPMISNPLPATVMPPAQGTPSAPAAATLSTVTSGDLDLFTE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 small ArfGAP 1
别名 Stromal membrane-associated protein 1, SMAP 1
计算分子量 467 aa, 50 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC036123
基因名称 SMAP1
Gene ID (NCBI) 60682
RRIDAB_2272651
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IYB5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMAP1, also named as FLJ13159 and SMAP 1, contains a Arf-GAP domain. SMAP1 is a GTPase activating protein for ARF6 that binds clathrin and regulates clathrin-dependent endocytosis. SMAP1 may play a role in erythropoiesis. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SMAP1.

实验方案

Product Specific Protocols
WB protocol for SMAP1 antibody 12799-1-APDownload protocol
IHC protocol for SMAP1 antibody 12799-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols