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验证数据展示

  • WB analysis of NIH/3T3 using 12799-1-AP

    WB analysis of NIH/3T3 using 12799-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12799-1-AP (SMAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 12799-1-AP

    IHC staining of human stomach using 12799-1-AP

    Immunohistochemical analysis of paraffin-embedded normal stomach slide using 12799-1-AP (SMAP1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SMAP1 Polyclonal antibody

SMAP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12799-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Stromal membrane-associated protein 1, SMAP 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, human adrenal gland tissue, human plasma, mouse brain tissue, rat lymph tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12799-1-AP targets SMAP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SMAP1 fusion protein Ag3544 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 small ArfGAP 1
别名 Stromal membrane-associated protein 1, SMAP 1
计算分子量 467 aa, 50 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC036123
基因名称 SMAP1
Gene ID (NCBI) 60682
RRIDAB_2272651
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IYB5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMAP1, also named as FLJ13159 and SMAP 1, contains a Arf-GAP domain. SMAP1 is a GTPase activating protein for ARF6 that binds clathrin and regulates clathrin-dependent endocytosis. SMAP1 may play a role in erythropoiesis. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SMAP1.

实验方案

Product Specific Protocols
WB protocol for SMAP1 antibody 12799-1-APDownload protocol
IHC protocol for SMAP1 antibody 12799-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols