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SLITRK1 Polyclonal antibody

SLITRK1 Polyclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 21119-1-AP

Print datasheet

Synonyms

KIAA0918, KIAA1910, LRRC12, RP11 395N17.1, SLIT and NTRK like protein 1, SLITRK1

验证数据展示

  • WB analysis using 21119-1-AP

    WB analysis using 21119-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21119-1-AP (SLITRK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 21119-1-AP

    IHC staining of mouse brain using 21119-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21119-1-AP (SLITRK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 21119-1-AP

    IHC staining of mouse brain using 21119-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21119-1-AP (SLITRK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 21119-1-AP

    IF Staining of mouse brain using 21119-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SLITRK1 antibody (21119-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 21119-1-AP

    IF Staining of mouse brain using 21119-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SLITRK1 antibody (21119-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21119-1-AP targets SLITRK1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P, IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen SLITRK1 fusion protein Ag15461 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SLIT and NTRK-like family, member 1
Synonyms KIAA0918, KIAA1910, LRRC12, RP11 395N17.1, SLIT and NTRK like protein 1, SLITRK1
Calculated Molecular Weight 696 aa, 78 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC051738
Gene Symbol SLITRK1
Gene ID (NCBI) 114798
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96PX8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SLITRK1 antibody 21119-1-APDownload protocol
IHC protocol for SLITRK1 antibody 21119-1-APDownload protocol
IF protocol for SLITRK1 antibody 21119-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols