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验证数据展示

  • WB analysis using 26379-1-AP

    WB analysis using 26379-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26379-1-AP (SLC25A36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 26379-1-AP

    IF Staining of HepG2 using 26379-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SLC25A36 antibody (26379-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

SLC25A36 Polyclonal antibody

SLC25A36 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26379-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Solute carrier family 25 member 36

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26379-1-AP targets SLC25A36 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag23894

Product name: Recombinant human SLC25A36 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 97-184 aa of BC014064

Sequence: IYFAAYSNCKEKLNDVFDPDSTQVHMISAAMAGFTAITATNPIWLIKTRLQLDARNRGERRMGAFECVRKVYQTDGLKGFYRGMSASY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 25, member 36
别名 Solute carrier family 25 member 36
观测分子量32-34 kDa
GenBank蛋白编号BC014064
基因名称 SLC25A36
Gene ID (NCBI) 55186
RRIDAB_3669532
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96CQ1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC25A36 is a pyrimidine nucleotide carrier that belongs to the solute carrier family 25 (SLC25). SLC25A36 transports cytosine and uracil (deoxy)nucleosides monophosphate, diphosphate, and triphosphate via uniporter and antiporter(PMID: 25320081). It plays an important role in maintaining mitochondrial biogenesis. Loss of SLC25A36 in mouse embryonic stem cells (mESCs) is associated with mtDNA loss and mitochondrial dysfunction(PMID: 31036718).

实验方案

Product Specific Protocols
IF protocol for SLC25A36 antibody 26379-1-APDownload protocol
WB protocol for SLC25A36 antibody 26379-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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