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SIK1 Polyclonal antibody
SIK1 Polyclonal Antibody for IHC,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IHC,ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive IHC detected in | human testis tissue, human stomach tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
The immunogen of 17370-1-AP is SIK1 Fusion Protein expressed in E. coli.
| Tested Applications | IHC,ELISA |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | SIK1 fusion protein Ag10954 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | salt-inducible kinase 1 |
| Synonyms | MSK, salt inducible kinase 1, SIK, SIK 1, SIK1, SNF1LK |
| Calculated Molecular Weight | 783 aa, 85 kDa |
| GenBank Accession Number | BC038504 |
| Gene Symbol | SIK1 |
| Gene ID (NCBI) | 150094 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P57059 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SIK1(Salt-inducible kinase 1) is also named as SIK, SNF1LK and belongs to the CAMK Ser/Thr protein kinase family. It associates constitutively with the NK regulatory complex and is responsible for increases in its catalytic activity following small elevations in intracellular sodium concentrations(PMID:17939993). SIK1 is required for phosphorylation of class II HDACs and for expression of MEF2 target genes in myocytes(PMID:17468767). Defects in SIK1 may be associated with some cancers, such as breast cancers. Loss of SIK1 correlates with poor patient outcome in breast cancers(PMID:19622832).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SIK1 antibody 17370-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS One Salt-inducible kinases (SIK) inhibition reduces RANKL-induced osteoclastogenesis.
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