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SH2D4A Polyclonal antibody

SH2D4A Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 15957-1-AP

Print datasheet

Synonyms

Protein SH(2)A, SH2 domain containing 4A, SH2A, SH2D4A

验证数据展示

  • WB analysis of HeLa using 15957-1-AP

    WB analysis of HeLa using 15957-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15957-1-AP (SH2D4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15957-1-AP

    IF Staining of HeLa using 15957-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15957-1-AP (SH2D4A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15957-1-AP targets SH2D4A in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen SH2D4A fusion protein Ag8713 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SH2 domain containing 4A
Synonyms Protein SH(2)A, SH2 domain containing 4A, SH2A, SH2D4A
Calculated Molecular Weight 454 aa, 53 kDa
Observed Molecular Weight 48-50 kDa
GenBank Accession NumberBC014525
Gene Symbol SH2D4A
Gene ID (NCBI) 63898
RRIDAB_2286025
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H788
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SH2D4A antibody 15957-1-APDownload protocol
IF protocol for SH2D4A antibody 15957-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Aging (Albany NY)

Integrated clustering signature of genomic heterogeneity, stemness and tumor microenvironment predicts glioma prognosis and immunotherapy response

Authors - Yangyang Wu
  • KD Validated
View Article
humanIHC,WB

Cell Signal

SH2D4A inhibits esophageal squamous cell carcinoma progression through FAK/PI3K/AKT signaling pathway

Authors - Haoming Shi
View Article