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验证数据展示

  • WB analysis of HeLa using 15957-1-AP

    WB analysis of HeLa using 15957-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15957-1-AP (SH2D4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 15957-1-AP

    IHC staining of human stomach cancer using 15957-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15957-1-AP (SH2D4A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 15957-1-AP

    IF Staining of HeLa using 15957-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SH2D4A antibody (15957-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

SH2D4A Polyclonal antibody

SH2D4A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15957-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

SH2A, SH2 domain-containing protein 4A, Protein phosphatase 1 regulatory subunit 38, PPP1R38

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15957-1-AP targets SH2D4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag8713

Product name: Recombinant human SH2D4A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-338 aa of BC014525

Sequence: HSEEFTNSLKTKSQYHDLQAPDNQQTKDIWKKVAEKEELEQGSRPAPTLEEEKIRSLSSSSRNIQQMLADSINRMKAYAFHQKKESMKKKQDEEINQIEEERTKQICKSWKEDSEWQASLRKSKAADEKRRSLAKQAREDYKRLSLGAQKGRGGERLQNPLRVPQKPERPPLPPKPQFLNSGAYPQKPLRNQGVVRTLSSSAQEDIIRWFKEEQLPLRAGYQKTSDTIAPWFHGILTLKKANELLLSTGMPGSFLIRVSERIKGYALSYLSEDGCKHFLIDASADAYSFLGVDQLQHATLADLVEYHKEEPITSLGKELLLYPCGQQDQLPDYLELFE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SH2 domain containing 4A
别名 SH2A, SH2 domain-containing protein 4A, Protein phosphatase 1 regulatory subunit 38, PPP1R38
计算分子量 454 aa, 53 kDa
观测分子量 48-50 kDa
GenBank蛋白编号BC014525
基因名称 SH2D4A
Gene ID (NCBI) 63898
RRIDAB_2286025
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H788
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for SH2D4A antibody 15957-1-APDownload protocol
IHC protocol for SH2D4A antibody 15957-1-APDownload protocol
WB protocol for SH2D4A antibody 15957-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,WB

Cell Signal

SH2D4A inhibits esophageal squamous cell carcinoma progression through FAK/PI3K/AKT signaling pathway

Authors - Haoming Shi
View Article
humanWB,IHC

Aging (Albany NY)

Integrated clustering signature of genomic heterogeneity, stemness and tumor microenvironment predicts glioma prognosis and immunotherapy response

Authors - Yangyang Wu
  • KD Validated
View Article
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