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验证数据展示

  • WB analysis of HeLa using 15957-1-AP

    WB analysis of HeLa using 15957-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15957-1-AP (SH2D4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 15957-1-AP

    IHC staining of human stomach cancer using 15957-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15957-1-AP (SH2D4A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 15957-1-AP

    IF Staining of HeLa using 15957-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SH2D4A antibody (15957-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

SH2D4A Polyclonal antibody

SH2D4A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15957-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

SH2A, SH2 domain-containing protein 4A, Protein phosphatase 1 regulatory subunit 38, PPP1R38

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15957-1-AP targets SH2D4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原 SH2D4A fusion protein Ag8713 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SH2 domain containing 4A
别名 SH2A, SH2 domain-containing protein 4A, Protein phosphatase 1 regulatory subunit 38, PPP1R38
计算分子量 454 aa, 53 kDa
观测分子量 48-50 kDa
GenBank蛋白编号BC014525
基因名称 SH2D4A
Gene ID (NCBI) 63898
RRIDAB_2286025
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H788
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SH2D4A antibody 15957-1-APDownload protocol
IHC protocol for SH2D4A antibody 15957-1-APDownload protocol
IF protocol for SH2D4A antibody 15957-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Aging (Albany NY)

Integrated clustering signature of genomic heterogeneity, stemness and tumor microenvironment predicts glioma prognosis and immunotherapy response

Authors - Yangyang Wu
  • KD Validated
View Article
humanIHC,WB

Cell Signal

SH2D4A inhibits esophageal squamous cell carcinoma progression through FAK/PI3K/AKT signaling pathway

Authors - Haoming Shi
View Article