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验证数据展示

  • WB analysis using 30867-1-AP

    WB analysis using 30867-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30867-1-AP (SGOL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 30867-1-AP

    IHC staining of mouse testis using 30867-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30867-1-AP (SGOL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 30867-1-AP

    IF Staining of A431 using 30867-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SGOL2 antibody (30867-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

SGOL2 Polyclonal antibody

SGOL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30867-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

FLJ25211, SGO2, SGOL2, Shugoshin 2, Shugoshin like 2, shugoshin like 2 (S. pombe), TRIPIN

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHT-1080 cells, HeLa cells, U2OS cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30867-1-AP targets SGOL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 SGOL2 fusion protein Ag29656 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 shugoshin-like 2 (S. pombe)
别名 FLJ25211, SGO2, SGOL2, Shugoshin 2, Shugoshin like 2, shugoshin like 2 (S. pombe), TRIPIN
计算分子量 1265 aa, 145 kDa
观测分子量180 kDa
GenBank蛋白编号BC092412
基因名称 SGOL2
Gene ID (NCBI) 151246
RRIDAB_3669770
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ562F6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SGOL2 (Shugoshin 2, also known as SGO2), which is tightly involved in the cell cycle process, has been reported to protect cohesion, sustain the linkage of chromosomes, and regulate kinetochore-microtubule attachment in meiosis and mitosis (PMID: 21273764). SGOL2 has been reported as a differentially expressed gene in various types of cancer, including glioma, hepatocellular cancer, and endometrial cancer (PMID: 36566018).

实验方案

Product Specific Protocols
WB protocol for SGOL2 antibody 30867-1-APDownload protocol
IHC protocol for SGOL2 antibody 30867-1-APDownload protocol
IF protocol for SGOL2 antibody 30867-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols