验证数据展示
经过测试的应用
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22100-1-AP targets SGK3 in IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag17308 Product name: Recombinant human SGK3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 371-496 aa of BC015326 Sequence: DNILHKPLSLRPGVSLTAWSILEELLEKDRQNRLGAKEDFLEIQNHPFFESLSWADLVQKKIPPPFNPNVAGPDDIRNFDTAFTEETVPYSVCVSSDYSIVNASVLEADDAFVGFSYAPPSEDLFL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | serum/glucocorticoid regulated kinase family, member 3 |
| 别名 | CISK, SGK3, SGKL |
| 计算分子量 | 496 aa, 57 kDa |
| 观测分子量 | 42 kDa, 57 kDa |
| GenBank蛋白编号 | BC015326 |
| 基因名称 | SGK3 |
| Gene ID (NCBI) | 23678 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q53EW6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Serum- and glucocorticoid-inducible kinase 3 (SGK3) is a protein kinase of the AGC family of protein kinase A, protein kinase G, and protein kinase C and functions downstream of phosphatidylinositol 3-kinase (PI3K)(PMID:21084382). It is also named as DKFZp781N0293, CISK, SGK2, SGKL and may be involved in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. SGK3 mediates cell IL-3-dependent survival signals (PMID:12397388). SGK3 has 2 isoforms produced by alternative splicing with the molecular weight of 57 kDa and 53 kDa and there is a isoform X3 can be detected with 378 amino acids(Sequence ID: ref|XP_005251262.1|).
