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验证数据展示

  • WB analysis of HeLa using 12699-1-AP (same clone as 12699-1-PBS)

    WB analysis of HeLa using 12699-1-AP (same clone as 12699-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 12699-1-AP (same clone as 12699-1-PBS)

    IHC staining of human pancreas cancer using 12699-1-AP (same clone as 12699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12699-1-AP (SGK3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse heart using 12699-1-AP (same clone as 12699-1-PBS)

    IP experiment of mouse heart using 12699-1-AP (same clone as 12699-1-PBS)

    IP result of anti-SGK3 (IP:12699-1-AP, 4ug; Detection:12699-1-AP 1:500) with mouse heart tissue lysate 3200ug. This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

  • IF Staining of A375 using 12699-1-AP (same clone as 12699-1-PBS)

    IF Staining of A375 using 12699-1-AP (same clone as 12699-1-PBS)

    Immunofluorescent analysis of A375 cells using 12699-1-AP (SGK3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

SGK3 Polyclonal antibody, PBS Only

SGK3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12699-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

12699-1-PBS targets SGK3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3149

Product name: Recombinant human SGK3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 160-496 aa of BC015326

Sequence: TDFDFLKVIGKGSFGKVLLAKRKLDGKFYAVKVLQKKIVLNRKEQKHIMAERNVLLKNVKHPFLVGLHYSFQTTEKLYFVLDFVNGGELFFHLQRERSFPEHRARFYAAEIASALGYLHSIKIVYRDLKPENILLDSVGHVVLTDFGLCKEGIAISDTTTTFCGTPEYLAPEVIRKQPYDNTVDWWCLGAVLYEMLYGLPPFYCRDVAEMYDNILHKPLSLRPGVSLTAWSILEELLEKDRQNRLGAKEDFLEIQNHPFFESLSWADLVQKKIPPPFNPNVAGPDDIRNFDTAFTEETVPYSVCVSSDYSIVNASVLEADDAFVGFSYAPPSEDLFL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serum/glucocorticoid regulated kinase family, member 3
别名
计算分子量 496 aa, 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC015326
基因名称 SGK3
Gene ID (NCBI) 23678
RRIDAB_2188548
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ53EW6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Serum- and glucocorticoid-inducible kinase 3 (SGK3) is a protein kinase of the AGC family of protein kinase A, protein kinase G, and protein kinase C and functions downstream of phosphatidylinositol 3-kinase (PI3K)(PMID:21084382). It is also named as DKFZp781N0293, CISK, SGK2, SGKL and may be involved in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. SGK3 mediates cell IL-3-dependent survival signals (PMID:12397388). SGK3 has 2 isoforms produced by alternative splicing with the molecular weight of 57 kDa and 53 kDa.

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