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SFRS8 Polyclonal antibody

SFRS8 Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 24705-1-AP

Print datasheet

Synonyms

SFRS8, SWAP

验证数据展示

  • WB analysis of Jurkat using 24705-1-AP

    WB analysis of Jurkat using 24705-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24705-1-AP (SFRS8 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 24705-1-AP

    IF Staining of HeLa using 24705-1-AP

    Immunofluorescent analysis of HeLa cells using 24705-1-AP (SFRS8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24705-1-AP is SFRS8 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen SFRS8 fusion protein Ag19762 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name splicing factor, arginine/serine-rich 8 (suppressor-of-white-apricot homolog, Drosophila)
Synonyms SFRS8, SWAP
Calculated Molecular Weight 951 aa, 105 kDa
Observed Molecular Weight 130-150 kDa
GenBank Accession NumberBC136678
Gene Symbol SFRS8
Gene ID (NCBI) 6433
RRIDAB_2879682
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12872
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SFRS8, also named as SFSWAP and SWAP, is splicing regulator. It regulates the splicing of fibronectin and CD45 genes. SFRS8 represses the splicing of MAPT/Tau exon 10. This antibody detects 130-150 kDa SFRS8 protein.

实验方案

Product Specific Protocols
WB protocol for SFRS8 antibody 24705-1-APDownload protocol
IF protocol for SFRS8 antibody 24705-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

Clin Transl Med

Splicing factor arginine/serine-rich 8 promotes multiple myeloma malignancy and bone lesion through alternative splicing of CACYBP and exosome-based cellular communication.

Authors - Yuanjiao Zhang
View Article